Meiotic segregation analysis in cows carrying the t(1;29) Robertsonian translocation
Meiotic segregation analysis in cows carrying the t(1;29) Robertsonian translocation
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DOI：
10.1159/000118744
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发表时间：
2008-01-01
影响因子：
1.7
通讯作者：
Ducos, A.
中科院分区：
文献类型：
--
作者：
Bonnet-Garnier, A.;Lacaze, S.;Ducos, A.
罗伯逊易位的杂合子携带者通常具有正常的表型，但存在生殖失败。在牛中，t（1;29）罗伯逊易位非常常见，携带者的生育力下降3-5%。有资料表明，女性携带者有较高的下降比男性携带者，但没有直接的研究，卵母细胞的染色体含量从t（1;29）载体母牛已进行到目前为止，4杂合子携带母牛进行激素刺激和卵泡穿刺，约800个卵母细胞体外成熟。600中期II制备和牛1号染色体和29绘画探针荧光原位杂交分析。不同类型卵母细胞的比例：正常平衡卵占74.11%（292/394），不平衡卵占4.06%（16/394），二倍体卵占21.83%（86/394）。所有奶牛的正常卵母细胞数与易位卵母细胞数差异不显著，但二倍体和不平衡率差异显著。正如在公牛中发现的那样，母牛的减数分裂分离模式显示出交替产物的优势。然而，在雌性中确定的不平衡配子的频率（4.06%）显著高于在雄性中观察到的频率（2.76%）。二倍体配子率的差异（0.04%对21.83%）主要是由雄性和雌性之间的差异解释的。版权所有（C）2008 S. Karger AG，巴塞尔。
Heterozygous carriers of Robertsonian translocations generally have a normal phenotype but present reproductive failure. In cattle, the t(1;29) Robertsonian translocation is very common and carriers show a 3-5% decrease in fertility. Some data suggest that female carriers have a higher decrease than male carriers but no direct studies of the chromosome content of oocytes from a t(1;29) carrier cow have been performed so far.Four heterozygous carrier cows underwent hormonal stimulations and follicles punctions and about 800 oocytes were matured in vitro. Six hundred metaphase II preparations were obtained and analysed by fluorescent in situ hybridization with bovine chromosome 1 and 29 painting probes. Proportions of different kinds of oocytes were assessed: 74.11% (292/394) were normal and balanced, 4.06% (16/394) unbalanced and 21.83% (86/394) diploid. For all cows, the number of normal oocytes was not significantly different from the number of translocated oocytes but the diploidy and unbalanced rate were significantly different between them. As found in bulls, the meiotic segregation pattern in cows has shown a preponderance of alternate products. However, the frequency of unbalanced gametes determined in females (4.06%) was significantly higher than the frequency observed in males (2.76%). The divergence in the rate of diploid gametes (0.04% vs. 21.83%) is mainly explained by the difference between males and females. Copyright (C) 2008 S. Karger AG, Basel.