Balanced GABAergic and glutamatergic synapse development in hippocampal neurons.
Balanced GABAergic and glutamatergic synapse development in hippocampal neurons.
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DOI：
10.1016/j.bbrc.2005.03.083
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发表时间：
2005-05
影响因子：
3.1
通讯作者：
Xi Zhao;S. Shoji;P. Lau
中科院分区：
文献类型：
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作者：
Xi Zhao;S. Shoji;P. Lau
兴奋性和抑制性突触的协调发展对于神经元回路的正常功能至关重要。使用海马神经元的同时和异时培养，我们比较了在不同阶段的海马能和GABA能突触的形成，并询问树突的年龄是否影响其接受新的海马能和GABA能突触的能力。用CFP-actin作为树突标记物或GFP-突触素作为突触前标记物转染神经元。我们发现，GFP-突触体素簇形成的CFP-肌动蛋白标记的树突在类似的密度，无论前和突触后细胞类型或树突的年龄（0-2周）后，共同培养。因此，成熟树突的年龄并不影响它们接受新突触的能力。由于GABA能传递在这些培养物中在1-2周内从去极化转换为超极化，我们的观察也表明这种发育转换不会改变GABA能突触的形成。
Coordinated development of excitatory and inhibitory synapses is crucial for normal function of neuronal circuits. Using homo- and heterochronic cultures of hippocampal neurons, we compared the formation of glutamatergic and GABAergic synapses at different stages and asked whether the age of dendrites affects their ability to accept new glutamatergic and GABAergic synapses. Neurons were transfected with either CFP-actin as a dendritic marker or GFP-synaptophysin as a presynaptic marker. We found that GFP-synaptophysin clusters formed on CFP-actin-labeled dendrites at similar density regardless of pre- and postsynaptic cell type or the age of dendrites (0–2 weeks) upon co-culturing. Therefore, the age of mature dendrites does not affect their ability to accept new synapses. Because GABAergic transmission switches from depolarizing to hyperpolarizing during 1–2 weeks in these cultures, our observations also suggest that this developmental switch does not alter the formation of GABAergic synapses.