Organ identity specification factor WGE localizes to the histone locus body and regulates histone expression to ensure genomic stability in Drosophila
Organ identity specification factor WGE localizes to the histone locus body and regulates histone expression to ensure genomic stability in Drosophila
复制标题
器官身份规范因子 WGE 定位于组蛋白基因座并调节组蛋白表达以确保果蝇基因组稳定性
DOI：
10.1111/gtc.12354
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发表时间：
2016
期刊：
影响因子：
2.1
通讯作者：
Shoichiro Kurata
中科院分区：
文献类型：
--
作者：
Nao Ozawa;Hirofumi Furuhashi;Keita Masuko;Eriko Numao;Takashi Makino;Tamaki Yano;Shoichiro Kurata
果蝇眼成虫盘中有翅眼（WGE）的过表达诱导眼到翅的转化。内源性WGE是器官发育所必需的，并且WGE缺陷突变体在幼虫阶段表现出生长停滞，这表明WGE对正常生长至关重要。然而，两性平等问题工作组的职能仍不清楚。在这里，我们分析了WGE的亚细胞定位，以了解其内源性功能。免疫染色显示，WGE定位于称为组蛋白基因座体（HLB）的特定核灶，这是S期特异性组蛋白mRNA产生所需的进化上保守的核体。组蛋白mRNA水平和蛋白水平在胞质组分中异常上调，提示WGE在调节组蛋白基因表达中的作用。遗传分析表明，WGE抑制位置效应杂色，并且WGE和HLB组分Mute似乎协同参与异染色质形成。进一步支持在染色质调节中的作用，wge缺陷突变体显示逆转录转座子的去阻遏和增加的γH2Av信号，这是一种DNA损伤标记。这些结果表明，WGE是果蝇HLB的一个组成部分，在异染色质形成和转座子沉默中起作用。我们认为，在HLB值的WGE有助于基因组的稳定性和发展，通过调节异染色质结构通过组蛋白基因调控。
Over‐expression of Winged‐Eye (WGE) in theDrosophilaeye imaginal disc induces an eye‐to‐wing transformation. Endogenous WGE is required for organ development, andwge‐deficient mutants exhibit growth arrest at the larval stage, suggesting that WGE is critical for normal growth. The function of WGE, however, remains unclear. Here, we analyzed the subcellular localization of WGE to gain insight into its endogenous function. Immunostaining showed that WGE localized to specific nuclear foci called the histone locus body (HLB), an evolutionarily conserved nuclear body required for S phase‐specific histone mRNA production. Histone mRNA levels and protein levels in cytosolic fractions were aberrantly up‐regulated inwgemutant larva, suggesting a role for WGE in regulatinghistonegene expression. Genetic analyses showed thatwgesuppresses position‐effect variegation, and that WGE and a HLB component Mute appears to be synergistically involved in heterochromatin formation. Further supporting a role in chromatin regulation,wge‐deficient mutants showed derepression of retrotransposons and increased γH2Av signals, a DNA damage marker. These findings suggest that WGE is a component of HLB inDrosophilawith a role in heterochromatin formation and transposon silencing. We propose that WGE at HLB contributes to genomic stability and development by regulating heterochromatin structure viahistonegene regulation.