Isolation and identification of a potent allelopathic substance in rice root exudates
Isolation and identification of a potent allelopathic substance in rice root exudates
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DOI：
10.1034/j.1399-3054.2002.1150310.x
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发表时间：
2002-07-01
影响因子：
6.4
通讯作者：
Yamamura, S
中科院分区：
文献类型：
--
作者：
Kato-Noguchi, H;Ino, T;Yamamura, S
为了阐明水稻化感作用系统，对水稻根系分泌物中的生长抑制物质进行了研究。水稻幼苗对水芹（Lepidium sativum L.）和莴苣（Lactuca sativa L.）当水芹和莴苣与水稻幼苗一起生长时，从水稻幼苗培养液中分离出一种对水稻幼苗有抑制作用的化合物，经光谱分析确定其化学结构为苦内酯B。苦瓜内酯B在浓度分别大于3 μ M和30 μ M时抑制水芹和莴苣幼苗的生长。在去壳和不去壳水稻幼苗的培养液中，豆内酯B的浓度分别为3.4和1.1 nmol/苗。这些结果表明，水稻幼苗可能向环境中释放豆蔻内酯B，并且稻壳处理引起的胁迫可能增加豆蔻内酯B的释放量。因此，豆蔻内酯B可能在水稻化感作用中起重要作用。
A search for growth inhibitors in rice root exudates was undertaken in order to clarify the allelopathic system in rice (Oryza sativa L.). Rice seedlings inhibited the growth of cress (Lepidium sativum L.) and lettuce (Lactuca sativa L.) seedlings when the cress and lettuce were grown with rice seedlings. The putative compound causing the inhibitory effect of rice seedlings was isolated from their culture solution, and the chemical structure of the inhibitor was determined by spectral data as momilactone B. Momilactone B inhibited the growth of cress and lettuce seedlings at concentrations greater than 3 and 30 muM , respectively. The concentration of momilactone B was 3.4 and 1.1 nmol per seedling in the culture solutions of husked and non-husked rice seedlings, respectively. These results suggest that rice seedlings may release momilactone B into the environment and the stress caused by the husk-treatment may increase the amount of momilactone B released. Thus, momilactone B may play an important role in rice allelopathy.