Meta-analytic method reveal a significant association of theBDNF Val66Met variant with smoking persistence based on a large samples
Meta-analytic method reveal a significant association of theBDNF Val66Met variant with smoking persistence based on a large samples
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基于大样本的荟萃分析方法揭示了 BDNF Val66Met 变异与吸烟持续性的显着关联
DOI：
10.1038/s41397-019-0124-y
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发表时间：
2020-06-01
影响因子：
2.8
通讯作者：
Li, Lijuan
中科院分区：
文献类型：
--
作者：
Zhao, Hailong;Xiong, Shuicai;Li, Lijuan
尽管许多遗传学研究报道了BDNF基因中的Val 66 Met与吸烟之间的联系，但这一发现仍然存在争议，主要是由于小到中等的样本量。本研究的主要目的是探讨Val 66 Met变异体在吸烟持续性过程中是否具有遗传功能。以Val为基础的显性遗传模型将瓦尔/*（即瓦尔/瓦尔+瓦尔/Met）和Met/Met作为两种基因型，并比较各基因型在当前吸烟者和从不吸烟者中的频率。本次Meta分析共收集了7篇遗传相关性文献，包括8个独立的数据集，10,160例受试者。鉴于种族对研究同质性的潜在影响，我们使用广泛使用的Comprehensive Meta分析软件（V2.0），根据祖先来源进行了单独的Meta分析。我们的荟萃分析结果表明，Val 66 Met多态性与吸烟持续性显著相关，无论是基于所有选择的样本，（N = 10，160;随机和固定模型：合并OR = 1.23; 95% CI = 1.03-1.46; P值= 0.012）或亚洲样本（N = 2，095;固定模型：合并OR = 1.25; 95% CI = 1.01-1.54; P值= 0.044;随机模型：合并OR = 1.25; 95% CI = 1.001-1.56; P值= 0.049）。未检测到出版物偏倚或研究间异质性的显著线索。因此，我们得出结论，Val 66 Met（rs6265）变异传递了保持吸烟的遗传易感性，携带瓦尔/* 基因型的吸烟者比携带Met/Met基因型的吸烟者保持吸烟的可能性更高。
Although numerous genetic studies have reported the link between Val66Met in BDNF gene with smoking, the findings remain controversial, mainly due to small-to-moderate sample sizes. The main aim of current investigation is to explore whether the variant of Val66Met has any genetic functions in the progress of smoking persistence. The Val-based dominant genetic model considering Val/* (namely, Val/Val + Val/Met) and Met/Met as two genotypes with comparison of the frequency of each genotype in current smokers and never smokers. There were seven genetic association articles including eight independent datasets with 10,160 participants were chosen in current meta-analytic investigation. In light of the potent effects of ethnicity on homogeneity across studies, we carried out separated meta-analyses according to the ancestry origin by using the wide-used tool of Comprehensive Meta-analysis software (V 2.0). Our meta-analyses results indicated that the Val66Met polymorphism was significantly linked with smoking persistence based on either all the chosen samples (N = 10,160; Random and fixed models: pooled OR = 1.23; 95% CI = 1.03-1.46; P value = 0.012) or Asian samples (N = 2,095; Fixed model: pooled OR = 1.25; 95% CI = 1.01-1.54; P value = 0.044; Random model: pooled OR = 1.25; 95% CI = 1.001-1.56; P value = 0.049). No significant clue of bias in publications or heterogeneity across studies was detected. Thus, we conclude that the Val66Met (rs6265) variant conveys genetic susceptibility to maintaining smoking, and smokers who carry Val/* genotypes have a higher possibility of maintaining smoking than those having Met/Met genotype.