Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum
Integrated Proteomics and Metabolomics Analysis Provides Insights into Ganoderic Acid Biosynthesis in Response to Methyl Jasmonate in Ganoderma Lucidum
复制标题
综合蛋白质组学和代谢组学分析提供了对灵芝中茉莉酸甲酯响应的灵芝酸生物合成的见解。
DOI：
10.3390/ijms20246116
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发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Zhao, Ming-Wen
中科院分区：
文献类型：
--
作者：
Jiang, Ai-Liang;Liu, Yong-Nan;Zhao, Ming-Wen
灵芝是公认的药用担子菌。茉莉酸甲酯（MeJA）能诱导灵芝的主要活性成分灵芝酸（GAs）的生物合成。lucidum。但监管机制仍不清楚。本研究采用整合蛋白质组学和代谢组学的方法，对G. lucidum中的蛋白质和代谢物的差异，以全面确定在MeJA处理15分钟（M15）和24小时（M24）。我们的研究成功地鉴定了M15中的209个差异丰度蛋白（DAP）和M24中的202个DAP。我们还在M24中通过GC-MS鉴定了154种代谢物，通过LC-MS鉴定了70种代谢物，这些代谢物参与了几种代谢途径。通过深入分析，我们发现了一些参与氧化还原过程、次生代谢、能量代谢、转录和翻译调控以及蛋白质合成的DAP及其代谢产物。特别是，我们的研究结果表明，MeJA治疗导致代谢重排，抑制正常的葡萄糖代谢，能量供应和蛋白质合成的细胞，但促进次级代谢产物，包括GAs。总之，我们的蛋白质组学和代谢组学数据进一步证实了MeJA对G.为进一步研究赤灵芝中MeJA信号应答和GA生物合成的分子机制提供了宝贵的资源。灵芝和其他相关物种。
Ganoderma lucidum is widely recognized as a medicinal basidiomycete. It was previously reported that the plant hormone methyl jasmonate (MeJA) could induce the biosynthesis of ganoderic acids (GAs), which are the main active ingredients of G. lucidum. However, the regulatory mechanism is still unclear. In this study, integrated proteomics and metabolomics were employed on G. lucidum to globally identify differences in proteins and metabolites under MeJA treatment for 15 min (M15) and 24 h (M24). Our study successfully identified 209 differential abundance proteins (DAPs) in M15 and 202 DAPs in M24. We also identified 154 metabolites by GC-MS and 70 metabolites by LC-MS in M24 that are involved in several metabolic pathways. With an in-depth analysis, we found some DAPs and metabolites that are involved in the oxidoreduction process, secondary metabolism, energy metabolism, transcriptional and translational regulation, and protein synthesis. In particular, our results reveal that MeJA treatment leads to metabolic rearrangement that inhibited the normal glucose metabolism, energy supply, and protein synthesis of cells but promoted secondary metabolites, including GAs. In conclusion, our proteomics and metabolomics data further confirm the promoting effect of MeJA on the biosynthesis of GAs in G. lucidum and will provide a valuable resource for further investigation of the molecular mechanisms of MeJA signal response and GA biosynthesis in G. lucidum and other related species.