Is saliva as reliable as urine for detection of cytomegalovirus DNA for neonatal screening of congenital CMV infection?
Is saliva as reliable as urine for detection of cytomegalovirus DNA for neonatal screening of congenital CMV infection?
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DOI：
10.1016/j.jcv.2006.03.011
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发表时间：
2006-07-01
影响因子：
8.8
通讯作者：
Coelho, Thalita Bonadio
中科院分区：
文献类型：
--
作者：
Yamamoto, Aparecida Yulie;Mussi-Pinhata, Marisa Marcia;Coelho, Thalita Bonadio
背景资料：有没有研究的检测巨细胞病毒（CMV）DNA的分子方法在新生儿的唾液中的大规模screeningprogrammes.Objectives：为了评估唾液作为一个样本的有用性，为新生儿先天性巨细胞病毒感染的筛查相比，尿液处理时，由PCR.Study Design：唾液和尿液样本，在生命的第一周内获得。这两种样本均试图从前2816名新生儿中获得。随后，仅从其他1623名婴儿中获得唾液。尿液和唾液经DNA-PCR检测。阳性结果的确认是通过PCR和病毒分离出生后3 wk完成的。结果：893/2816（31.7%）的婴儿无法获得尿样。唾液和尿液样本均来自剩余的1923名婴儿。其中CMV感染者28例（1.45%）。两种样品的结果之间的一致性为99.7%。当PCR应用于尿液（1.3%）或唾液（1.2%）样本时，CMV排泄相似。在随后的1623名婴儿，其中只有唾液样本计划进行筛查，16（0.98%）是CMV-infected.Conclusions：唾液样本是有用的，尿液中的CMV-DNA的鉴定，在大规模使用的筛选程序。(c)2006 Elsevier B. V.保留所有权利。
Background: There are no studies on the detection of cytomegalovirus (CMV) DNA by molecular methods in the saliva of newborn infants in large scale screening programs.Objectives: To evaluate the usefulness of saliva as a sample for the neonatal screening of congenital CMV infection as compared to urine when processed by a PCR.Study Design: Saliva and urine samples were obtained during the first week of life. Both samples were attempted to be obtained from the first 2816 neonates. Subsequently, only saliva was obtained from other 1623 infants. Urine and saliva were processed by DNA-PCR. Confirmation of positive results was done by PCR and virus isolation by 3 weeks after birth.Results: A urine sample was not obtainable from 893/2816 (31.7%) infants. Both saliva and urine samples were obtained from the remaining 1923 infants. Of these, 28 (1.45%) were CMV-infected. There was 99.7% agreement between the results with both samples. CMV excretion was similar when PCR was applied to urine (1.3%) or to saliva (1.2%) samples. Among the subsequent 1623 infants for whom only a saliva sample was planned for screening, 16 (0.98%) were CMV-infected.Conclusions: Saliva samples are as useful as urine for the identification of CMV-DNA in large use for screening programs. (c) 2006 Elsevier B.V. All rights reserved.