T-CELLS MIGRATE TO TUMOR SITES AFTER EXTRACORPOREAL INTERLEUKIN-2 STIMULATION AND REINFUSION IN A PATIENT WITH METASTATIC MELANOMA
T-CELLS MIGRATE TO TUMOR SITES AFTER EXTRACORPOREAL INTERLEUKIN-2 STIMULATION AND REINFUSION IN A PATIENT WITH METASTATIC MELANOMA
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DOI：
10.1111/j.1365-2133.1993.tb00198.x
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发表时间：
1993-04-01
影响因子：
10.3
通讯作者：
BURG, G
中科院分区：
文献类型：
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作者：
DUMMER, R;BECKER, JC;BURG, G
通过白细胞分离术从黑色素瘤皮肤转移患者身上获取外周血单核细胞 (PBMC)，并使用 1000 IU 重组白细胞介素 2 (IL-2)/ml 进行体外刺激，产生淋巴因子激活的杀伤细胞 (LAK 细胞)。双色免疫荧光分析表明，IL-2 诱导自然杀伤细胞 (CD56+) 和 T 淋巴细胞 (CD3-) 上 CD25 上调。用铟-111进行放射性标记后，将细胞回输。伽马相机成像显示肿瘤部位富集。闪烁扫描之前和之后对肿瘤组织进行的免疫染色显示了 CD25-T 淋巴细胞（CD2+、CD3+）。但没有自然杀伤细胞（CD16+、CD56+）浸润转移灶。体内黑色素瘤转移灶处的 LAK 细胞富集不涉及自然杀伤细胞，但其特征是该患者中活化的 T 淋巴细胞数量增加。
Peripheral blood mononuclear cells (PBMC) were taken by leukapheresis from a patient with melanoma skin metastases and stimulated in vitro using 1000 IU recombinant interleukin 2 (IL-2)/ml to generate lymphokine-activated killer cells (LAK cells). Two-colour immunofluorescence analysis demonstrated an IL-2-induced up-regulation of CD25 on natural killer cells (CD56+) as well as on T lymphocytes (CD3-). After radiolabelling with indium-111, the cells were reinfused. Gamma-camera imaging revealed an enrichment at the tumour sites. Immunostaining of tumour tissue taken before and after scintigraphy demonstrated CD25- T lymphocytes (CD2+, CD3+). but no natural killer cells (CD16+, CD56+) infiltrating the metastases.LAK cell enrichment at melanoma metastases in vivo did not involve natural killer cells, but was characterized by increased numbers of activated T lymphocytes in this patient.