Genomic DNA amplification by the multiple displacement amplification (MDA) method
Genomic DNA amplification by the multiple displacement amplification (MDA) method
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DOI：
10.1042/bst0370450
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发表时间：
2009-04-01
影响因子：
3.9
通讯作者：
Lasken, Roger S.
中科院分区：
文献类型：
--
作者：
Lasken, Roger S.
在检测分析和基因组分析中经常需要大量的DNA。DNA的有限可用性可能是满足研究和临床需求的关键障碍。通常需要DNA扩增方法从小样本或具有低DNA含量的环境样本中产生足够的材料。MDA（多重置换扩增）反应越来越多地成为许多应用的选择方法，因为其广泛覆盖基因组，与旧的全基因组扩增方法相比产生极长的DNA产物，并且即使从极少量的起始材料也具有高的DNA产率。值得注意的是，MDA甚至能够从单个微生物细胞进行基因组测序。MDA的一些用途及其优势和局限性将被讨论。
Large amounts of DNA are frequently required for use in detection assays and genomic analysis. The limited availability of DNA can be a critical obstacle to meeting research and clinical needs. DNA amplification methods are often required to generate sufficient material from small specimens or environmental samples with low DNA content. The MDA (multiple displacement amplification) reaction is increasingly the method of choice for many applications because of its extensive coverage of the genome, the generation of extremely long DNA products compared with older whole genome amplification methods and the high DNA yields, even from exceedingly low amounts of starting material. Remarkably, MDA enables genomic sequencing even from single microbial cells. Some of the uses of MDA and its strengths and limitations will be discussed.