Prevalence and genetic diversity of Bartonella species isolated from wild rodents in Japan
Prevalence and genetic diversity of Bartonella species isolated from wild rodents in Japan
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DOI：
10.1128/aem.00071-08
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发表时间：
2008-08-01
影响因子：
4.4
通讯作者：
Kawabata, Hiroki
中科院分区：
文献类型：
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作者：
Inoue, Kai;Maruyama, Soichi;Kawabata, Hiroki
在这里，我们描述了第一次在日本的野生啮齿动物巴尔通体生物体的患病率和遗传特性。我们在日本全国（12个县）捕获了685只野生啮齿动物，并成功地从685只啮齿动物中的176只（分离率，25.7%）分离出巴尔通体。从郊区捕获的啮齿动物中分离到的巴尔通体占51.8%，而从市区捕获的动物中未分离到巴尔通体。rpo B和gltA的序列分析显示，获得的巴尔通体分离物分为8个遗传群，包括与B密切相关的分离物。grahamii（A-I组）、B. tribocorum和B. elizabethae（B-J群），B. tribocorum和B. rattimassiliensis（C-K群）、B. rattimassiliensis（D-L组）、B. phoceensis（F-N组）、B. taylorii（G-O组），以及可能另外两个新的巴尔通体属种组（E-M和H-P）。B。grahamii是人类神经性视网膜炎的潜在病原体之一，在日本啮齿类动物中占优势。就这些巴尔通体遗传群与其啮齿动物种属之间的关系而言，（i）A-I、E-M和H-P群似乎与美丽姬鼠和银色姬鼠有关;（ii）C-K、D-L和F-N群可能与大鼠有关;（iii）B-J群似乎与姬鼠和大鼠有关。rattus;（iv）G-O群可能与A. speciosus和Clethrionomys田鼠。此外，在A. speciosus和R.老鼠这些发现表明，日本的啮齿动物可能是人畜共患巴尔通体感染的宿主。
Here, we describe for the first time the prevalence and genetic properties of Bartonella organisms in wild rodents in Japan. We captured 685 wild rodents throughout Japan (in 12 prefectures) and successfully isolated Bartonella organisms from 176 of the 685 rodents (isolation rate, 25.7%). Those Bartonella isolates were all obtained from the rodents captured in suburban areas (rate, 51.8%), but no organism was isolated from the animals captured in city areas. Sequence analysis of rpoB and gltA revealed that the Bartonella isolates obtained were classified into eight genetic groups, comprising isolates closely related to B. grahamii (A-I group), B. tribocorum and B. elizabethae (B-J group), B. tribocorum and B. rattimassiliensis (C-K group), B. rattimassiliensis (D-L group), B. phoceensis (F-N group), B. taylorii (G-O group), and probably two additional novel Bartonella species groups (E-M and H-P). B. grahamii, which is one of the potential causative agents of human neuroretinitis, was found to be predominant in Japanese rodents. In terms of the relationships between these Bartonella genetic groups and their rodent species, (i) the A-I, E-M, and H-P groups appear to be associated with Apodemus speciosus and Apodemus argenteus; (ii) the C-K, D-L, and F-N groups are likely implicated in Rattus rattus; (iii) the B-J group seems to be involved in Apodemus mice and R. rattus; and (iv) the G-O group is probably associated with A. speciosus and Clethrionomys voles. Furthermore, dual infections with two different genetic groups of bartonellae were found in A. speciosus and R. rattus. These findings suggest that the rodent in Japan might serve as a reservoir of zoonotic Bartonella infection.