Cytotoxic evaluation of essential oil from Casearia sylvestris Sw on human cancer cells and erythrocytes
Cytotoxic evaluation of essential oil from Casearia sylvestris Sw on human cancer cells and erythrocytes
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DOI：
10.1590/s0044-59672008000100012
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发表时间：
2008-01-01
期刊：
影响因子：
0.8
通讯作者：
Yano, Tomomasa
中科院分区：
文献类型：
--
作者：
Silva, Saulo Luís da;Chaar, Jamal da Silva;Yano, Tomomasa
Casearia sylvestris（Flacourtiaceae）是一种俗称“guacatonga”的植物，南美洲（巴西、秘鲁和玻利维亚）的土著居民使用它来治疗包括癌症在内的多种疾病。细胞毒性研究表明，由于酪蛋白的存在，它具有有趣的抗肿瘤潜力，并且精油显示出高比例的强效细胞毒性。 倍半萜烯（P-石竹烯和et-葎草烯）。在这项工作中，我们证实，与非肿瘤细胞Vero (CD 50 210.1 mu)相比，樟子松精油对HeLa、A-549和HT-29肿瘤细胞(分别为CD 50 63.3、60.7和90.6 mu g.ml(-1))具有良好的选择性细胞毒性。 g.ml(-1))和小鼠巨噬细胞(CD 50 234.0 mu g.ml(-1))。该油会导致七种不同红细胞溶血，表明樟子松必须小心使用。此外，还测试了P-石竹烯和α-葎草烯的标准品，它们表现出与油中存在的细胞毒性相似的细胞毒性，表明它们 可能是本研究中观察到的毒性作用的原因。
Casearia sylvestris (Flacourtiaceae) is a plant popularly known as "guacatonga' and it is used by indigenous population from South America (Brazil, Peru and Bolivia) in the treatment of several diseases, including cancer. Cytotoxic studies showed that it presents an interesting antitumoral potential due to the presence of casearins and the essential oil showed a high percentage of potent cytotoxic sesquiterpenes (P-caryophyllene and et-humulene). In this work, we verified that the essential oil of C sylvestris presents a good selective cytotoxicity against HeLa, A-549 and HT-29 tumor cells (CD 50 63.3, 60.7 and 90.6 mu g.ml(-1), respectively) when compared to non-tumoral cells Vero (CD 50 210.1 mu g.ml(-1)) and mice macrophages (CD 50 234.0 mu g.ml(-1)). The oil causes hemolysis in seven different kinds of erythrocytes, indicating that C sylvestris must be used carefully. Besides, standard of P-caryophyllene and a-humulene were also tested and they showed similar cyrotoxicity to the cytotoxicity presented in the oil, indicating that they might be responsible for the toxic effects that were observed in this study.