Sensory innervation of the periodontal ligament in the incisor and molar of the monkey, Macaca fuscata. An immunohistochemical study for neurofilament protein and glia-specific S-100 protein.
Sensory innervation of the periodontal ligament in the incisor and molar of the monkey, Macaca fuscata. An immunohistochemical study for neurofilament protein and glia-specific S-100 protein.
复制标题
猴子门牙和臼齿牙周韧带的感觉神经支配，Macaca fuscata。
DOI：
10.1679/aohc.50.437
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Maeda
中科院分区：
文献类型：
--
作者：
T. Maeda
应用神经丝蛋白（NFP）和S-100蛋白免疫组织化学方法，对恒河猴牙周膜内的神经成分进行了研究。厚的神经束与NFP-免疫反应进入牙周膜通过槽窝底部的狭缝，而较薄的束，也NFP-免疫反应，穿透韧带从侧壁的槽窝。NFP免疫阳性神经纤维在切牙和磨牙的根尖周围密集分布。在上切牙，另一个密集分布的NFP阳性神经被发现在舌侧牙周膜的顶侧和唇侧牙周膜的冠侧。切牙中的牙周神经纤维以树枝状的方式分叉，终止于牙周膜内的游离神经末梢。S-100蛋白免疫染色显示神经的许旺氏鞘，其分布模式与门牙牙周膜中NFP免疫阳性纤维的分布模式相同。在磨牙中，NFP和S-100的免疫染色显示，神经在牙周膜的顶端区域是众多的。然而，在磨牙的根尖区，NFP免疫阳性神经少于S-100阳性神经元。这种差异表明存在NFP免疫阴性神经。因此，S-100的免疫染色是有用的演示神经元在磨牙牙周膜。S-100免疫反应阳性神经元在牙周膜中的数量在切牙比在磨牙。磨牙牙周膜内除游离神经末梢外，还存在少量卷曲神经末梢。三叉神经节内的神经纤维和胞体大多呈NFP阳性反应，而三叉神经中脑核内的神经纤维和胞体均呈阴性反应。这一发现表明磨牙牙周膜接受三叉神经节和中脑核的双重神经支配。
Nervous elements in the periodontal ligament of the monkey, Macaca fuscata were investigated by means of immunohistochemistry for neurofilament protein (NFP) and S-100 protein. Thick nerve bundles with NFP-immunoreactivity entered the periodontal ligament through slits at the bottom of the alveolar socket, whereas thinner bundles, also NFP-immunoreactive, penetrated the ligament from the lateral wall of the alveolar socket. The NFP-immunopositive nerve fibers were densely distributed around the root apex in both incisors and molars. In the upper incisors, another dense distribution of NFP-positive nerves was found in the apical half of the lingual periodontal ligament and in the coronal half of the labial periodontal ligament. The periodontal nerve fibers in the incisors ramified in a dendritic fashion, terminating as free nerve endings within the periodontal ligament. Immunostaining for S-100 protein demonstrated the Schwann sheaths of nerves which showed the same distribution pattern as that of the NFP-immunopositive fibers in the periodontal ligament of the incisors. In molars, immunostaining for NFP and S-100 showed that nerves were numerous in the apical region of the periodontal ligament. However, NFP-immunopositive nerves were fewer than the S-100-positive nervous elements in the apical region in molars. This discrepancy suggests the presence of NFP-immunonegative nerves. Immunostaining for S-100 is thus useful for demonstration of neural elements in the periodontal ligament of molars. The S-100-immunoreactive neural elements in the periodontal ligament were more numerous in incisors than in molars. In addition to the free nerve endings, a small population of coiled nerve endings was observed in the periodontal ligament of molars. Most of the nerve fibers and cell bodies in the trigeminal ganglion showed NFP-immunoreactivity, whereas all in the mesencephalic nucleus of the trigeminal nerve were negative in NFP-reaction. This finding suggests that the periodontal ligament of molars receives a dual innervation from the trigeminal ganglion and mesencephalic nucleus.