Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain
Involvement of spinal orexin A in the electroacupuncture analgesia in a rat model of post-laparotomy pain
复制标题
脊髓食欲素A参与电针镇痛大鼠剖腹术后疼痛模型
DOI：
10.1186/1472-6882-12-225
复制
发表时间：
2012-11-22
影响因子：
--
通讯作者：
Wu, Gen-Cheng
中科院分区：
文献类型：
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作者：
Feng, Xiao-Ming;Mi, Wen-Li;Wu, Gen-Cheng
背景食欲素A（OXA，hypocretin/hcrt 1）是一种新发现的潜在镇痛物质。然而，OXA是否参与针刺镇痛仍不清楚。本研究旨在探讨脊髓OXA参与电针镇痛（EA）。使用Von Frey细丝测量后爪和腹部的机械性异常性疼痛。围术期分别以2/15 Hz或2/100 Hz电针双侧ST 36和SP 6各1次，每次30 min。结果OXA（0.3nmol）和2/15 Hz电针对模型均有镇痛作用（P<0.05），OXA（0.3nmol）和2/15 Hz电针（0.3nmol）对模型的镇痛作用明显优于OXB（0.05）。术前鞘内注射SB-334867 30 nmol可拮抗OXA的镇痛作用，减弱EA的镇痛作用（P<0.05）。SB-334867对芬太尼镇痛无明显阻断作用（P>0.05）。阿片受体拮抗剂纳洛酮不能拮抗OXA的镇痛作用（P>0.05）。
BackgroundOrexin A (OXA, hypocretin/hcrt 1) is a newly discovered potential analgesic substance. However, whether OXA is involved in acupuncture analgesia remains unknown. The present study was designed to investigate the involvement of spinal OXA in electroacupuncture (EA) analgesia.MethodsA modified rat model of post-laparotomy pain was adopted and evaluated. Von Frey filaments were used to measure mechanical allodynia of the hind paw and abdomen. EA at 2/15 Hz or 2/100 Hz was performed once on the bilateral ST36 and SP6 for 30 min perioperatively. SB-334867, a selective orexin 1 receptor (OX1R) antagonist with a higher affinity for OXA than OXB, was intrathecally injected to observe its effect on EA analgesia.ResultsOXA at 0.3 nmol and EA at 2/15 Hz produced respective analgesic effects on the model (P<0.05). Pre-surgical intrathecal administered of SB-334867 30 nmol antagonized OXA analgesia and attenuated the analgesic effect of EA (P<0.05). However, SB-334867 did not block fentanyl-induced analgesia (P>0.05). In addition, naloxone, a selective opioid receptor antagonist, failed to antagonize OXA-induced analgesia (P>0.05).ConclusionsThe results of the present study indicate the involvement of OXA in EA analgesia via OX1R in an opioid-independent way.