Metaproteomics: A new approach for studying functional microbial ecology
Metaproteomics: A new approach for studying functional microbial ecology
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DOI：
10.1007/s00248-006-9196-8
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发表时间：
2007-04-01
影响因子：
3.6
通讯作者：
Lemanceau, Philippe
中科院分区：
文献类型：
--
作者：
Maron, Pierre-Alain;Ranjard, Lionel;Lemanceau, Philippe
在后基因组时代，人们清楚地认识到基于核酸的方法在获得原位微生物群落表达的功能信息方面的局限性。在这方面，对土著微生物群落表达的蛋白质（元蛋白质组）的大规模研究应提供信息，以深入了解生态系统中微生物组成部分的功能。元蛋白质组的表征有望为微生物群落的遗传和功能多样性提供数据。对元蛋白质组、元基因组和元转录组的研究将有助于我们对微生物群落及其在生态系统功能中的贡献的认识。由于目前正在进行的环境测序项目，随着越来越多的宏基因组信息的提供，元蛋白质组学方法的有效性将得到提高。更具体地说，在对比的环境条件下的元蛋白质组分析应该允许（1）跟踪新的功能基因和代谢途径和（2）识别蛋白质优先与特定的压力。这些被视为功能性生物指示剂的蛋白质今后应有助于帮助决策者确定可持续管理我们环境的战略。
In the postgenomic era, there is a clear recognition of the limitations of nucleic acid-based methods for getting information on functions expressed by microbial communities in situ. In this context, the large-scale study of proteins expressed by indigenous microbial communities (metaproteome) should provide information to gain insights into the functioning of the microbial component in ecosystems. Characterization of the metaproteome is expected to provide data linking genetic and functional diversity of microbial communities. Studies on the metaproteome together with those on the metagenome and the metatranscriptome will contribute to progress in our knowledge of microbial communities and their contribution in ecosystem functioning. Effectiveness of the metaproteomic approach will be improved as increasing metagenomic information is made available thanks to the environmental sequencing projects currently running. More specifically, analysis of metaproteome in contrasted environmental situations should allow (1) tracking new functional genes and metabolic pathways and (2) identifying proteins preferentially associated with specific stresses. These proteins considered as functional bioindicators should contribute, in the future, to help policy makers in defining strategies for sustainable management of our environment.