Relevance of biofilms in pediatric tonsillar disease
Relevance of biofilms in pediatric tonsillar disease
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DOI：
10.1007/s10096-011-1249-3
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发表时间：
2011-12-01
影响因子：
4.5
通讯作者：
Paglini-Oliva, P.
中科院分区：
文献类型：
--
作者：
Diaz, R. R.;Picciafuoco, S.;Paglini-Oliva, P.
在这项研究中，我们研究了慢性炎症的扁桃体，临床特征，并在隐窝中的生物膜的存在与阻塞性肥大和复发性上呼吸道病变的患者之间的关系。36例因阻塞性原因需要接受扁桃体切除术的患者（年龄1至6岁），其中所有患者在手术前30天均未服用任何抗生素。用苏木精-伊红和革兰氏染色、荧光显微镜和共聚焦激光显微镜检查样品。主要症状与梗阻性病变有关，而非感染（p < 0.01）。所有患者均有扁桃体肥大（III级或IV级），但66.66%的病例与腺样体肥大相关（p < 0.05）。77.28%的扁桃体隐窝内存在生物膜，但生物膜的存在与否与扁桃体肥大和扁桃体滤泡的数量无关。在这里，我们证明了像刺耳的沙哑的声音，扁桃体和腺样体肥大，呼吸暂停和颈部腺病的症状显然与扁桃体中生物膜的存在有关。我们的研究结果使我们能够提出生物膜参与扁桃体和腺样体肥大的发病机制。应在早期阶段重点预防生物膜的形成，试图抑制细菌附着在呼吸道粘膜上。
In this investigation, we study the relation between chronic inflammation of the tonsils, clinical features, and the presence of biofilms in the crypts in patients presenting with obstructive hypertrophy and recurrent upper airway pathology. Thirty-six patients who needed to undergo a tonsillectomy for obstructive reasons (aged 1 to 6 years), among which none of them had taken any antibiotics 30 days prior to surgery, were included. Samples were examined with hematoxylin-eosin and Gram staining, fluorescent microscopy, and confocal laser microscopy. The predominance of symptoms were those related to obstructive pathology rather than infection (p < 0.01). All patients had tonsillar hypertrophy (grade III or IV), but an association with adenoids hypertrophy was detected in 66.66% of cases (p < 0.05). 77.28% of tonsils presented biofilms in their crypts, but hypertrophy and tonsillar follicle number were not related to the presence or absence of biofilms. Here, we demonstrated that symptoms like harsh raucous sound, tonsillar and adenoids hypertrophy, apnea, and cervical adenopathies are clearly related to the presence of biofilm in tonsils. Our results allow us to propose that biofilms are involved in the pathogenesis of tonsils and adenoids hypertrophy. The prevention of biofilms formation should be focused in the early stages, attempting to restrain bacterial attachment to the respiratory mucosa.