Stimulation of sexual behavior in the male rat by galanin-like peptide
Stimulation of sexual behavior in the male rat by galanin-like peptide
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DOI：
10.1016/j.yhbeh.2004.04.008
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发表时间：
2004-12-01
影响因子：
3.5
通讯作者：
Clifton, DK
中科院分区：
文献类型：
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作者：
Fraley, GS;Thomas-Smith, SE;Clifton, DK
甘丙肽样肽（GALP）是最近描述的一种神经肽，其与甘丙肽具有部分序列同一性，但源自单独的基因。中枢注射 GALP 会刺激促性腺激素释放激素 (GnRH) 和黄体生成素 (LH) 的分泌，并诱导已知调节大鼠雄性性行为的几个大脑区域中 Fos 的表达。我们推测 GALP 也可能与其对下丘脑-垂体-性腺 (HPG) 轴的刺激作用一起刺激性行为。为了检验这一假设，我们将 GALP、甘丙肽或载体（人工脑脊液，aCSF）单独给予有性经验的雄性大鼠，并评估这些药物对性行为的影响。我们观察到，与单独的 aCSF 相比，GALP 显着增加了男性典型性行为的各个方面，而甘丙肽则抑制所有这些相同的行为。为了检查 GALP 对性行为的刺激作用是否是由 GALP 对 HPG 轴的刺激作用介导的，我们阉割了相同的雄性大鼠并重复了行为实验。我们发现 GALP 在去势动物中保持了其对雄性典型性行为的诱导作用，这表明 GALP 对性行为的影响并不是 GALP 刺激睾酮分泌的能力的结果。这些观察结果表明 GALP 神经元是控制雄性大鼠性行为的下丘脑回路的一部分。 (C) 2004 Elsevier Inc. 保留所有权利。
Galanin-like peptide (GALP) is a recently described neuropeptide, which shares a partial sequence identity with galanin but is derived from a separate gene. Central injections of GALP stimulate the secretion of gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) and induce the expression of Fos in several brain areas known to regulate male sexual behavior in the rat. We postulated that GALP may also stimulate sexual behavior in concert with its stimulatory effect on the hypothalamic-pituitary-gonadal (HPG) axis. To test this hypothesis, we administered GALP, galanin, or the vehicle (artificial cerebrospinal fluid, aCSF) alone to sexually experienced male rats and assessed the effects of these agents on sexual behavior. We observed that compared to aCSF alone, GALP significantly increased all aspects of male-typical sexual behavior, whereas galanin inhibited all of these same behaviors. To examine whether the stimulatory effects of GALP on sexual behavior were mediated by GALP's stimulatory effects on the HPG axis, we castrated the same male rats and repeated the behavioral experiment. We found that GALP maintained its inductive action on male-typical sexual behaviors in the castrated animals, suggesting that the effects of GALP on sexual behavior are not the result of GALP's ability to stimulate testosterone secretion. These observations suggest that GALP neurons are part of the hypothalamic circuitry controlling sexual behavior in the male rat. (C) 2004 Elsevier Inc. All rights reserved.