ADP-Ribosylation factors do not activate yeast phospholipase Ds but are required for sporulation.
ADP-Ribosylation factors do not activate yeast phospholipase Ds but are required for sporulation.
复制标题
ADP-核糖基化因子不会激活酵母磷脂酶 D，但是孢子形成所必需的。
DOI：
10.1091/mbc.9.8.2025
复制
发表时间：
1998
影响因子：
3.3
通讯作者：
Engebrecht,J
中科院分区：
文献类型：
--
作者：
Rudge,SA;Cavenagh,MM;Kamath,R;Sciorra,VA;Morris,AJ;Kahn,RA;Engebrecht,J
ADP-ribosylation factor (ARF) proteins inSaccharomyces cerevisiaeare encoded by two genes,ARF1andARF2. The addition of the c-myc epitope at the C terminus of Arf1 resulted in a mutant (arf1-myc arf2) that supported vegetative growth and rescued cells from supersensitivity to fluoride, but homozygous diploids failed to sporulate.arf1-myc arf2mutants completed both meiotic divisions but were unable to form spores. TheSPO14gene encodes a phospholipase D (PLD), whose activity is essential for mediating the formation of the prospore membrane, a prerequisite event for spore formation. Spo14 localized normally to the developing prospore membrane inarf1-myc arf2mutants; however, the synthesis of the membrane was attenuated. This was not a consequence of reduced PLD catalytic activity, because the enzymatic activity of Spo14 was unaffected in meioticarf1-myc arf2mutants. Although potent activators of mammalian PLD1, Arf1 proteins did not influence the catalytic activities of either Spo14 or ScPld2, a second yeast PLD. These results demonstrate thatARF1is required for sporulation, and the mitotic and meiotic functions of Arf proteins are not mediated by the activation of any known yeast PLD activities. The implications of these results are discussed with respect to current models of Arf signaling.
