Antimicrobial Peptides: Insights into Membrane Permeabilization, Lipopolysaccharide Fragmentation and Application in Plant Disease Control.
Antimicrobial Peptides: Insights into Membrane Permeabilization, Lipopolysaccharide Fragmentation and Application in Plant Disease Control.
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DOI：
10.1038/srep11951
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发表时间：
2015-07-06
影响因子：
4.6
通讯作者：
Bhunia A
中科院分区：
文献类型：
--
作者：
Datta A;Ghosh A;Airoldi C;Sperandeo P;Mroue KH;Jiménez-Barbero J;Kundu P;Ramamoorthy A;Bhunia A
近年来，细菌感染的多药耐药性增加，已成为人类健康和全球粮食安全的一个主要问题。合成抗菌肽（AMP）由于其广谱的抗菌活性，近年来作为传统抗生素的潜在替代品受到了广泛关注。这些肽还涉及植物病害控制，用于取代污染和危害环境和人类健康的常规处理方法。在这里，我们报告从头设计和抗菌研究的VG 16，一个16个残基的活性片段的登革病毒融合肽。我们的研究结果表明，VG 16 KRKP，一个无毒和无溶血性的类似物的VG 16，显示出显着的抗革兰氏阴性大肠杆菌的抗菌活性。大肠杆菌和植物病原菌X.和X. campestris，以及对人类真菌病原体C.白色念珠菌和C. grubii。VG 16 KRKP还能够抑制植物中的细菌性疾病进展。脂多糖中VG 16 KRKP的溶液NMR结构具有折叠构象，其具有通过具有延伸的N-和C-末端的芳香族-芳香族堆积相互作用稳定的中心定位的转向型结构。VG 16 KRKP的从头设计为开发用于治疗人类和植物感染的更有效的抗菌和抗内毒素肽提供了有价值的见解。
The recent increase in multidrug resistance against bacterial infections has become a major concern to human health and global food security. Synthetic antimicrobial peptides (AMPs) have recently received substantial attention as potential alternatives to conventional antibiotics because of their potent broad-spectrum antimicrobial activity. These peptides have also been implicated in plant disease control for replacing conventional treatment methods that are polluting and hazardous to the environment and to human health. Here, we report de novo design and antimicrobial studies of VG16, a 16-residue active fragment of Dengue virus fusion peptide. Our results reveal that VG16KRKP, a non-toxic and non-hemolytic analogue of VG16, shows significant antimicrobial activity against Gram-negative E. coli and plant pathogens X. oryzae and X. campestris, as well as against human fungal pathogens C. albicans and C. grubii. VG16KRKP is also capable of inhibiting bacterial disease progression in plants. The solution-NMR structure of VG16KRKP in lipopolysaccharide features a folded conformation with a centrally located turn-type structure stabilized by aromatic-aromatic packing interactions with extended N- and C-termini. The de novo design of VG16KRKP provides valuable insights into the development of more potent antibacterial and antiendotoxic peptides for the treatment of human and plant infections.
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影响因子：
--
作者：
Ghosh, Anirban;Datta, Aritreyee;Bhunia, Anirban
通讯作者：
Bhunia, Anirban
影响因子：
2.9
作者：
Bhattacharjya, Surajit;Domadia, Prerna N.;David, Sunil A.
通讯作者：
David, Sunil A.
DOI：
10.2174/1568005024605855
发表时间：
2002-03-01
期刊：
Current Drug Targets - Infectious Disorders
影响因子：
--
作者：
Hancock, R. E. W.;Patrzykat, A.
通讯作者：
Patrzykat, A.
影响因子：
2.9
作者：
Jiang, Ziqing;Vasil, Adriana I.;Hodges, Robert S.
通讯作者：
Hodges, Robert S.
影响因子：
3.4
作者：
Fernandez, David I.;Lee, Tzong-Hsien;Separovic, Frances
通讯作者：
Separovic, Frances