Dehydration converts DsbG crystal diffraction from low to high resolution
Dehydration converts DsbG crystal diffraction from low to high resolution
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DOI：
10.1016/s0969-2126(03)00005-4
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发表时间：
2003-02-01
期刊：
影响因子：
5.7
通讯作者：
Martin, JL
中科院分区：
文献类型：
--
作者：
Heras, B;Edeling, MA;Martin, JL
衍射质量的晶体对于蛋白质结构的晶体学研究是必不可少的，并且衍射差的晶体的生产通常被认为是该过程中的死胡同。在这里，我们展示了衍射差的DsbG晶体的显着改善，允许高分辨率的衍射数据测量。在脱水之前，晶体是易碎的，衍射图案是条纹状的，延伸到10埃分辨率。脱水后，有一个惊人的改善，衍射图案扩展到2埃分辨率。这个和其他最近的结果表明，脱水是一种简单，快速，廉价的方法，将质量差的晶体转化为衍射质量的晶体。
Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Angstrom resolution. After dehydration, there is a spectacular improvement, with the diffraction pattern extending to 2 Angstrom resolution. This and other recent results show that dehydration is a simple, rapid, and inexpensive approach to convert poor quality crystals into diffraction quality crystals.