Outbreak Investigation of NADC30-Like PRRSV in South-East China
Outbreak Investigation of NADC30-Like PRRSV in South-East China
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DOI：
10.1111/tbed.12530
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发表时间：
2016-10-01
影响因子：
4.3
通讯作者：
Tian, K.
中科院分区：
文献类型：
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作者：
Li, C.;Zhuang, J.;Tian, K.
为了解2015年我国东南地区猪繁殖与呼吸综合征病毒（PRRSV）的流行情况，开展了猪繁殖与呼吸综合征病毒（PRRSV）NADC 30样毒株的流行病学调查。除此前报道的6个省份外，又发现2个省份有NADC 30样PRRSV流行。系统发育分析表明，这些病毒分离物聚集在一个独立的分支，并共享高度的核苷酸相似性，NADC 30，2型PRRSV已在美国分离，在2008年。从猪肺泡巨噬细胞上成功分离到1株NADC 30样PRRSV，并对6周龄无特定病原猪进行了致病性研究。接种病毒的猪表现出典型的PRRSV临床症状，但所有猪在整个研究期间均存活，存活期为14天。尸检时，感染猪的肺部发生了PRRSV特异性间质性肺炎，并在肺样本中检测到病毒抗原。因此，我们的研究结果表明，NADC 30样PRRSV在中国广泛传播，并可能导致猪的临床疾病。
Epidemiological outbreak investigations were conducted on NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV) to investigate the prevalence of the disease in south-east China in 2015. Two more provinces were found to have NADC30-like PRRSV circulating besides previously reported six provinces. Phylogenetic analysis showed that these virus isolates were clustered in an independent branch and shared high nucleotide similarity to NADC30, a type 2 PRRSV that has been isolated in Unite States in 2008. One NADC30-like PRRSV strain from Henan province was successfully isolated on porcine alveolar macrophages and was tested on 6-week-old specific pathogen-free pigs for pathogenic study. The virus-inoculated pigs showed typical PRRSV clinical symptoms, but all pigs survived throughout the study with a period of 14 days. At necropsy, the lungs of infected pigs developed PRRSV-specific interstitial pneumonia, and virus antigen was detected in lung samples. Therefore, our results indicated NADC30-like PRRSV has widely spread in China and could cause clinical disease on pigs.