Purification and Some Properties of an Endo-β-1, 6-glucanase from Neurospora crassa
Purification and Some Properties of an Endo-β-1, 6-glucanase from Neurospora crassa
复制标题
粗糙脉孢菌 Endo-β-1, 6-葡聚糖酶的纯化及其一些性质
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
K. Matsuda
中科院分区：
文献类型：
--
作者：
N. Hiura;T. Nakajima;K. Matsuda
一种内切-β-1，6-葡聚糖酶（E.C. 3.2.1.75）从粗糙脉孢菌IFO-6 O 68的培养物滤液中纯化，通过在CM-赛璐珞、Con-A琼脂糖4 B和琼脂糖Cl-6 B上层析，然后进行制备亲和凝胶电泳。纯化的酶具有47，000的表观分子量。该酶活性的最适pH和最适温度分别为5.0和50 ℃。该酶作用于β-1，6-葡聚糖（Pustulan），生成一系列具有内型作用的龙胆寡糖，最后生成葡萄糖和龙胆二糖。该酶也能作用于N. crassa细胞壁β-葡聚糖，和少量的水解片段被释放，而细胞壁葡聚糖分子没有明显的变化。
An endo-β-1,6-glucanase (E.C. 3.2.1.75) was purified from the culture filtrate of Neurospora crassa IFO-6O68 by chromatographies on CM-cellulofine, Con-A Sepharose 4B, and Sepharose Cl-6B followed by preparative affinity gel electrophoresis. The purified enzyme had an apparent molecular weight of 47,000. The pH and temperature optima for the activity were 5.0 and 50°C. The enzyme acted on β-1,6-glucan (Pustulan) and yielded a series of gentio-oligosaccharides with endo- type action, and finally, glucose and gentiobiose were produced. The enzyme was also able to act on N. crassa cell wall β-glucan, and a small amount of hydrolysis fragments were liberated without apparent change of the cell wall glucan molecules.