Stability of miRNA in human urine supports its biomarker potential.
Stability of miRNA in human urine supports its biomarker potential.
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miRNA在人尿中的稳定性支持其生物标志物潜力。
DOI：
10.2217/bmm.13.44
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发表时间：
2013-08
影响因子：
2.2
通讯作者：
Weiss RH
中科院分区：
文献类型：
--
作者：
Mall C;Rocke DM;Durbin-Johnson B;Weiss RH
MiRNAs作为泌尿系统疾病的生物标志物已显示出很大的实用价值，然而，对它们在尿液中的稳定性缺乏严格的评估。在这里，我们评估尿液中miRNAs在临床相关储存程序下的稳定性。8名健康人提供了干净的捕获尿样，这些尿样在室温或4℃保存5天，或在-80℃下经历10次冻融循环，每种情况下，两种miRNAs，miR-16和miR-21，用实时定量聚合酶链式反应进行定量。所有条件都显示尿液中miRNAs具有惊人的稳定性：在10次冻融循环结束时，仍有23-37%的初始量；在室温下储存5天，仍有35%的初始量；在4℃时，仍有42%-56%的初始量。这两种miRNAs的降解速度也大致相同。在各种储存条件下，miRNAs在尿液中相对稳定，这支持了它们作为尿液生物标志物的用途。
miRNAs are showing utility as biomarkers in urologic disease, however, a rigorous evaluation of their stability in urine is lacking. Here, we evaluate the stability of miRNAs in urine under clinically relevant storage procedures. Eight healthy individuals provided clean catch urine samples that were stored at room temperature or at 4°C for 5 days, or subjected to ten freeze–thaw cycles at -80°C. For each condition, two miRNAs, miR-16 and miR-21, were quantitated by quantitative real-time PCR. All conditions demonstrated a surprising degree of stability of miRNAs in the urine: by the end of ten freeze–thaw cycles, 23–37% of the initial amount remained; over the 5-day period of storage at room temperature, 35% of the initial amount remained; and at 4°C, 42–56% of the initial amount remained. Both miRNAs also showed degradation at approximately the same rate. miRNAs are relatively stable in urine under a variety of storage conditions, which supports their utility as urinary biomarkers.
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影响因子：
6.4
作者：
Ganti, Sheila;Taylor, Sandra L.;Kim, Kyoungmi;Hoppel, Charles L.;Guo, Lining;Yang, Joy;Evans, Christopher;Weiss, Robert H.
通讯作者：
Weiss, Robert H.
影响因子：
2.1
作者：
Juan, David;Alexe, Gabriela;Liou, Louis S.
通讯作者：
Liou, Louis S.
DOI：
10.1152/ajprenal.00722.2009
发表时间：
2010-04-01
影响因子：
4.2
作者：
Taylor, Sandra L.;Ganti, Sheila;Weiss, Robert H.
通讯作者：
Weiss, Robert H.
DOI：
10.1073/pnas.0804549105
发表时间：
2008-07-29
影响因子：
11.1
作者：
Mitchell, Patrick S.;Parkin, Rachael K.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh
影响因子：
4.8
作者：
Fasanaro, Pasquale;D'Alessandra, Yuri;Martelli, Fabio
通讯作者：
Martelli, Fabio