DEVELOPMENTAL-STUDY ON CAPACITY OF RABBIT GRANULOSA-CELLS TO RESPOND TO TROPHIC HORMONES AND SECRETE PROGESTERONE INVITRO
DEVELOPMENTAL-STUDY ON CAPACITY OF RABBIT GRANULOSA-CELLS TO RESPOND TO TROPHIC HORMONES AND SECRETE PROGESTERONE INVITRO
复制标题
DOI：
10.1016/0012-1606(74)90124-9
复制
发表时间：
1974-01-01
影响因子：
2.7
通讯作者：
RYAN, KJ
中科院分区：
文献类型：
--
作者：
ERICKSON, GF;CHALLIS, JRG;RYAN, KJ
为确定未分化的兔颗粒细胞何时开始分泌孕酮，取新生兔(新生30日龄)的完整卵巢和60~600日龄的15 0~12 0 0μm卵泡的纯颗粒细胞分别加入人绒毛膜促性腺激素(HCG)、卵泡刺激素(LFSH)、二丁酰环磷腺苷(BU2 CAMP)、前列腺素E2(PGE2)、雌二醇17β(E2)体外培养6~10d，并设对照组。每隔2天收集培养液，用放射免疫法测定孕酮、雌酮和雌二醇-17β的含量。新生兔卵巢和颗粒细胞培养均不能自发分泌雌酮或雌二醇-17β，也不受促性腺激素、Bu-2 cAMP或前列腺素E_2的刺激。新生兔和7日龄对照卵巢不分泌孕酮，17和30日龄兔卵巢分泌。促性腺激素和Bu2 cAMP促进7日龄卵巢孕酮分泌，17、30日龄卵巢孕酮分泌增加5~10倍，而前列腺素E2和17雌二醇β无此作用。所有有腔卵泡(2 0 0~12 0 0μm)的颗粒细胞自发分泌孕酮，促性腺激素和Bu2 cAMP可使其产生10 0~10 0 0倍，而雌二醇17β和前列腺素E2则不能刺激其产生。这些结果表明，当初级卵泡发育成腔时，兔颗粒细胞具有分泌孕酮的能力，并提示分化过程涉及到对促性腺激素的反应能力的获得，可能是通过合成或揭开促性腺激素受体的作用。
To determine when undifferentiated rabbit granulosa cells first develop the capacity to secrete progesterone, pieces of intact ovaries from neonatal rabbits (newborn—30 days old) and pure granulosa cells from 150–1200 μm follicles at 60–600 days old were cultured in vitro for 6–10 days with human chorionic gonadotropin (HCG), Pergonal (LH FSH), dibutyryl cyslic AMP (Bu 2 CAMP), prostaglandin E 2, estradiol-17β, and as controls. The culture medium was collected every 2 days, and progesterone, estrone, and estradiol-17β were measured by radioimmunoassay. None of the neonatal ovaries or granulosa cell cultures secreted estrone or estradiol-17β spontaneously or in response to stimulation by gonadotropins, Bu 2 CAMP, or prostaglandin E 2. Control cultures of newborn and 7-day-old ovaries did not secrete progesterone, but ovaries from 17-and 30-day-old rabbits did. Gonadotropins and Bu 2 CAMP induced progesterone secretion in 7-day-old ovaries and stimulated its production 5-10-fold in ovaries at 17 and 30 days old, but prostaglandin E 2 and estradiol-17β were without effect. Granulosa cells from all antral follicles (200–1200 μm) secreted progesterone spontaneously, and its production was stimulated 100–1000-fold with gonadotropins and Bu 2 CAMP, but not with estradiol-17β or prostaglandin E 2. In contrast, granulosa cells from 100–150 μm preantral follicles from 200-day-old animals did not secrete progesterone under these culture conditions. These results demonstrate that rabbit granulosa cells differentiate the capacity to secrete progesterone at the time the primary follicle develops an antrum, and suggest the differentiation process involves the acquisition of the capacity to respond to gonadotropins perhaps by the synthesis or unmasking of gonadotropin receptors.