Cuticle thickness affects dynamics of volatile emission from petunia flowers
Cuticle thickness affects dynamics of volatile emission from petunia flowers
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DOI：
10.1038/s41589-020-00670-w
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发表时间：
2020-10-19
影响因子：
14.8
通讯作者：
Dudareva, Natalia
中科院分区：
文献类型：
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作者：
Liao, Pan;Ray, Shaunak;Dudareva, Natalia
植物角质层是挥发性有机化合物 (VOC) 释放到大气中的最后一道屏障，但人们对它在排放过程中的作用知之甚少。在这里，我们结合反向遗传和化学方法，证明角质层对 VOC 传质具有显着的抵抗力，充当 VOC 的接收器/浓缩器，从而保护细胞免受这些疏水性化合物潜在有毒的内部积累的影响。角质层厚度的减少对各个 VOC 的影响不同，具体取决于其挥发性，并导致其内部细胞重新分布、传质阻力源的变化和 VOC 合成的改变。这些结果表明，角质层不仅仅是VOC穿越的被动扩散屏障，而且作为整个VOC网络的组成部分，在排放过程中发挥着上述复杂的作用。植物角质层最初被认为充当被动扩散屏障。遗传和代谢分析表明，它也是挥发物的接收器/浓缩器，保护细胞免受这些疏水性化合物的毒性作用。
The plant cuticle is the final barrier for volatile organic compounds (VOCs) to cross for release to the atmosphere, yet its role in the emission process is poorly understood. Here, using a combination of reverse-genetic and chemical approaches, we demonstrate that the cuticle imposes substantial resistance to VOC mass transfer, acting as a sink/concentrator for VOCs and hence protecting cells from the potentially toxic internal accumulation of these hydrophobic compounds. Reduction in cuticle thickness has differential effects on individual VOCs depending on their volatility, and leads to their internal cellular redistribution, a shift in mass transfer resistance sources and altered VOC synthesis. These results reveal that the cuticle is not simply a passive diffusion barrier for VOCs to cross, but plays the aforementioned complex roles in the emission process as an integral member of the overall VOC network.The plant cuticle was initially thought to act as a passive diffusion barrier. Genetic and metabolic analysis reveals that it is also a sink/concentrator for volatiles protecting cells from toxic effects of these hydrophobic compounds.