Electric field-guided collective motility initiation of large epidermal cell groups.
Electric field-guided collective motility initiation of large epidermal cell groups.
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DOI：
10.1091/mbc.e22-09-0391
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发表时间：
2023-05-01
影响因子：
3.3
通讯作者：
Mogilner, Alex
中科院分区：
文献类型：
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作者：
Sun, Yaohui;Reid, Brian;Zhang, Yan;Zhu, Kan;Ferreira, Fernando;Estrada, Alejandro;Sun, Yuxin;Draper, Bruce W.;Yue, Haicen;Copos, Calina;Lin, Francis;Bernadskaya, Yelena Y.;Zhao, Min;Mogilner, Alex
最近的研究已经阐明了单细胞极化的机械力化学途径，但对黏附细胞组中集体运动的启动知之甚少。我们使用斑马鱼角质细胞群的电流定向分析、药理扰动、电场开关、粒子成像测速仪和细胞跟踪来显示大的细胞群在几分钟内开始向阴极移动。有趣的是，虽然PI3K抑制的单个电池偏向阳极，但抑制PI3K并不影响阴极定向的电池组迁移。我们观察到，对照组的阴极迁移细胞在前部最快，而PI3K抑制组的前部细胞最慢。当电场方向颠倒时，对照组和PI3K抑制组都迅速复极化，而在电场关闭后，组迁移继续进行。抑制肌球蛋白破坏了角质形成细胞群的凝聚力，并取消了电场反转时的集体方向性和转换方向的能力。我们的数据与一个模型一致，根据该模型，群中的细胞独立地感知电场，并且细胞的机械整合导致一致的群运动。
Recent research has elucidated mechanochemical pathways of single cell polarization, but much less is known about collective motility initiation in adhesive cell groups. We used galvanotactic assays of zebrafish keratocyte cell groups, pharmacological perturbations, electric field switches, particle imaging velocimetry, and cell tracking to show that large cell groups initiate motility in minutes toward the cathode. Interestingly, while PI3K-inhibited single cells are biased toward the anode, inhibiting PI3K does not affect the cathode-directed cell group migration. We observed that control groups had the fastest cathode-migrating cell at the front, while the front cells in PI3K-inhibited groups were the slowest. Both control and PI3K-inhibited groups rapidly repolarized when the electric field direction was reversed, and the group migration continued after the electric field was switched off. Inhibiting myosin disrupted the cohesiveness of keratocyte groups and abolished the collective directionality and ability to switch direction when the electric field is reversed. Our data are consistent with a model according to which cells in the group sense the electric field individually and mechanical integration of the cells results in coherent group motility.