Genome-wide identification and expression analysis of potato PYL gene family.
Genome-wide identification and expression analysis of potato PYL gene family.
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DOI：
10.3724/sp.j.1006.2022.14183
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发表时间：
2022-01-01
期刊：
影响因子：
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通讯作者：
Wen, G. H.
中科院分区：
文献类型：
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作者：
Jia, Xiao-Xia;Qi, En-Fang;Wen, G. H.
ABA作为一种重要的信号分子，通过其核心信号通路PYLs-PP2Cs-SnRK2s广泛调控植物的生长发育和胁迫响应过程。PYLs蛋白作为ABA信号转导的核心成分，具有不可替代的作用。为探索马铃薯PYL （PYR/PYL/RCARs）基因的进化和表达模式，从马铃薯全基因组‘DM-v 6.1’中鉴定出17个PYL基因，并对其分布、蛋白质理化性质、系统进化、结构特征及相关表达模式进行了分析。结果表明，17个StPYL基因不均匀分布在8条染色体上，氨基酸为163 ~ 231 aa，等电点为4.5 ~ 8.6，相对分子量为18.71 ~ 25.29 kD。根据基因结构和蛋白质系统发育特征，将StPYL家族成员划分为3个亚群。Motif 1存在于该家族的所有基因中，表明其在stpyl的进化中相对保守。基因表达模式显示，StPYL家族成员具有明显的组织表达特异性，除StPYL1在外源激素（BAP、ABA和IAA）和非生物胁迫（高温、盐和干旱）下上调外，其余StPYL基因均有功能分化，不同胁迫下的相对表达模式不同。本研究结果为进一步阐明马铃薯stpyl基因的功能奠定了理论基础。
As a key signalling molecule, abscisic acid (ABA) extensively regulates plant growth and development, and stress response processes through its core signalling pathway PYLs-PP2Cs-SnRK2s. As a core component of ABA signalling transduction, PYLs protein plays an irreplaceable role. In order to explore the evolution and expression pattern of PYL (PYR/PYL/RCARs) genes in potato, 17 PYL genes were identified from the whole potato genome 'DM-v 6.1', and their distribution, protein physical and chemical properties, system evolution, structural characteristics, and the relative expression patterns were analysed. The results showed that the 17 StPYL genes were unevenly distributed on 8 chromosomes, with amino acids of 163-231 aa, isoelectric points of 4.5-8.6, and relative molecular weights of 18.71-25.29 kD. According to the gene structure and protein phylogenetic characteristics, the members of the StPYL family were divided into three subgroups. Motif 1 existed in all genes in this family, indicating that it was relatively conserved in the evolution of StPYLs. Gene expression patterns showed that the members of the StPYL family had obvious tissue expression specificity, and except for the up-regulation of StPYL1 under exogenous hormones (BAP, ABA, and IAA) and abiotic stress (high temperature, salt and drought), the rest of the StPYL genes were functionally differentiated and their relative expression patterns were different under different stresses. The results of this study lay a theoretical foundation for further elucidating the function of StPYLs gene in potato.