Al(3+) -promoted fluoride accumulation in tea plants (Camellia sinensis) was inhibited by an anion channel inhibitor DIDS.
Al(3+) -promoted fluoride accumulation in tea plants (Camellia sinensis) was inhibited by an anion channel inhibitor DIDS.
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DOI：
10.1002/jsfa.7626
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发表时间：
2016-09
影响因子：
4.1
通讯作者：
Xianchen Zhang;Hong-jian Gao;Tianyuan Yang;Hong-Hong Wu-Hong;Yu-Mei Wang;X. Wan
中科院分区：
文献类型：
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作者：
Xianchen Zhang;Hong-jian Gao;Tianyuan Yang;Hong-Hong Wu-Hong;Yu-Mei Wang;X. Wan
茶树一般生长在富铝(Al)和富氟(F)的酸性土壤上。最近的一项研究表明，Al(3+)处理通过增加内源Ca(2+)和钙调蛋白(CaM)来促进茶树体内氟的积累。茶叶中高水平的氟不仅会损害茶叶的质量，而且可能会对经常喝茶的人构成健康风险。因此，寻找一些可能有助于控制酸性土壤(Al(3+))茶树体内氟积累的线索具有重要意义。结果二异硫氰基二苯乙烯-2，2-二磺酸(DIDS)显著抑制了Al(3+)促进茶树体内氟的积累。此外，与Al(3+)处理相比，Al(3+)+DIDS处理显著增加了茶树根部Ca(2+)的外流，降低了CaM的水平。此外，Al(3+)+DDS处理的茶树根膜电位去极化显著高于Al(3+)处理，总H(+)流出量和质膜H(+)-ATPase活性也显著高于Al(3+)处理。结论阴离子通道阻滞剂DIDS可抑制Al(3+)促进的茶树氟积累。Ca(2+)/CaM和膜电位去极化可能参与了这一过程。©2016中国化学工业学会。
BACKGROUND Generally, tea plants are grown in acid soil which is rich in aluminum (Al) and fluoride (F). A recent publication showed that pretreatment with Al(3+) promoted F accumulation in tea plants by increasing endogenous Ca(2+) and calmodulin (CaM). A high level of F in tea leaves not only impairs tea quality but also might pose a health risk for people drinking tea regularly. Therefore it is important to try to find some clues which might be beneficial in controlling F accumulation in tea plants grown in acid soil (Al(3+) ). RESULTS It was found that diisothiocyanostilbene-2,2-disulfonic acid (DIDS) significantly reduced Al(3+) -promoted F accumulation in tea plants. Additionally, Al(3+) plus DIDS treatment stimulated significantly higher Ca(2+) efflux and decreased the CaM level in tea roots compared with Al(3+) treatment. Besides, significantly higher depolarization of membrane potential was shown in tea roots treated with Al(3+) plus DIDS than in those treated with Al(3+) , as well as higher net total H(+) efflux and plasma membrane H(+) -ATPase activity. CONCLUSION Al(3+) -promoted F accumulation in tea plants was inhibited by an anion channel inhibitor DIDS. Ca(2+) /CaM and membrane potential depolarization may be the components involved in this process. © 2016 Society of Chemical Industry.