Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma
Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma
复制标题
DOI：
10.3892/or.2017.5561
复制
发表时间：
2017-05-01
期刊：
影响因子：
4.2
通讯作者：
Katakura, Akira
中科院分区：
文献类型：
--
作者：
Ohshima, Mitsuyoshi;Sugahara, Keisuke;Katakura, Akira
本研究的目的是使用毛细管电泳-质谱（CE-MS）唾液样本的代谢组学分析来表征日本口腔鳞状细胞癌（OSCC）患者的代谢系统。先前的研究显示，使用CE-MS的口腔鳞状细胞癌患者的唾液代谢组中的种族和肿瘤部位之间存在差异。在本研究中，从22名日本口腔鳞状细胞癌患者和21名健康对照中获得唾液，这些患者访问了日本东京牙科大学市川综合医院牙科，口腔颌面外科，采用CE-MS技术对两组唾液进行代谢物组学分析，共检测到499个代谢物峰。共有25种代谢物被揭示为区分OSCC患者和健康对照者的潜在标志物：胆碱、对羟基苯乙酸和2-羟基-4-甲基戊酸（P
The aim of the present study was to characterize the metabolic systems in Japanese patients with oral squamous cell carcinoma (OSCC) using capillary electrophoresis-mass spectrometry (CE-MS) metabolome analysis of saliva samples. A previous study showed variations among ethnicities and tumor sites in the saliva metabolome of patients with OSCC using CE-MS. In the present study, saliva was obtained from 22 Japanese patients with OSCC and from 21 healthy controls who visited the Department of Dentistry, Oral and Maxillofacial Surgery, Tokyo Dental Collage Ichikawa General Hospital, Tokyo, Japan, and all samples were subject to comprehensive quantitative metabolome analysis using CE-MS. A total of 499 metabolites were detected as CE-MS peaks in the saliva tested from the two groups. A total of 25 metabolites were revealed as potential markers to discriminate between patients with OSCC and healthy controls: Choline, p-hydroxyphenylacetic acid, and 2-hydroxy-4-methylvaleric acid (P