A DNA polymorphism from five inbred strains of the mouse identifies a functional class of domesticus-type Y chromosome that produces the same phenotypic distribution of gonadal hermaphrodites.
A DNA polymorphism from five inbred strains of the mouse identifies a functional class of domesticus-type Y chromosome that produces the same phenotypic distribution of gonadal hermaphrodites.
复制标题
来自五个近交系小鼠的 DNA 多态性鉴定出一种功能类的家蝇型 Y 染色体，它产生与性腺雌雄同体相同的表型分布。
作者：
F. Biddle;B. A. Eales;Y. Nishioka
家鼠的野生CLA近交系包含一个家养类型的Y染色体，该染色体缺少带有AC11探针片段1的2.3kb Taqi带。当与C57BL/6J品系回交时，Cla Y染色体也导致低频率的XY性腺两性(F·G·比德尔和Y.Nishioka，1988)。基因组，30：870-878)。在四个历史自交系AKR/J、MA/MyJ、PL/J和RF/J中发现了相似的家系Y染色体，缺少2.3kb的Taqi带。当回交到C57BL/6J时，这四条Y染色体导致类似CLA Y的性腺雌雄同体的频率很低，性腺类型的表型分布与CLA Y的相似。到目前为止，2.3kb Taqi带的缺失似乎是家系Y染色体的一种多态，它识别了三个功能类别中的一个。只能通过它们对C57BL/6J品系回交试验胎儿睾丸分化的影响来区分。另外三个历史上的近交系Bub/BNJ、ST/BJ和SWR/J也被检测到，它们具有家族型Y染色体，但包含2.3kb的Taqi带。他们允许C57BL/6J回交测试胎儿正常的睾丸发育。
The wild-derived CLA inbred strain of the house mouse contains a domesticus-type Y chromosome that lacks a 2.3-kb TaqI band with fragment 1 of the AC11 probe. The CLA Y chromosome also causes a low frequency of XY gonadal hermaphrodites when backcrossed to the C57BL/6J strain (F. G. Biddle and Y. Nishioka, 1988. Genome, 30:870-878). A similar domesticus-type Y chromosome, lacking the 2.3-kb TaqI band has now been found in the four historical inbred strains AKR/J, MA/MyJ, PL/J, and RF/J. When backcrossed to C57BL/6J, these four Y chromosomes cause low frequencies of gonadal hermaphrodites similar to the CLA Y and phenotypic distribution of types of gonad are indistinguishable from that with the CLA Y. The absence of the 2.3-kb TaqI band appears to be a polymorphism among domesticus-type Y chromosomes that identifies one of the three functional classes that, so far, can be distinguished only by their effects on testis differentiation in backcross test fetuses with the C57BL/6J strain. Three other historical inbred strains, BUB/BnJ, ST/bJ, and SWR/J, with a domesticus-type Y chromosome but containing the 2.3-kb TaqI band, were also assayed. They permit normal testis development in backcross test fetuses with C57BL/6J.