The telomeric transcriptome of Schizosaccharomyces pombe.
The telomeric transcriptome of Schizosaccharomyces pombe.
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DOI：
10.1093/nar/gkr1153
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发表时间：
2012-04
影响因子：
14.9
通讯作者：
Azzalin CM
中科院分区：
文献类型：
--
作者：
Bah A;Wischnewski H;Shchepachev V;Azzalin CM
真核细胞端粒被转录成含有端粒重复序列的RNA（TERRA）。端粒转录在哺乳动物、鸟类、斑马鱼、植物和芽殖酵母中都有报道。在这里，我们表明，裂殖酵母的染色体末端产生不同的RNA种类。与芽殖酵母和哺乳动物一样，S.粟酒裂殖酵母含有富含G的TERRA分子和以与TERRA相反的方向转录的亚端粒RNA种类（ARRET）。此外，裂殖酵母染色体末端产生两种新的RNA种类：富含C的端粒重复序列转录物（ARIA）和与ARRET互补的亚端粒转录物（αARRET）。RNA聚合酶II（RNAPII）在体内与粟酒裂殖酵母染色体末端相关联，并且端粒因子Rap1负调节这种关联，以及来自染色体末端的RNA的细胞积累。我们还发现RNAPII亚基Rpb7和非典型的poly（A）聚合酶Cid12和Cid14参与了TERRA、ARIA、ARRET和αARRET转录的调节。我们证实了端粒转录的进化保守性，并揭示了模式生物之间端粒转录本的组成和调节的有趣相似性和差异。
Eukaryotic telomeres are transcribed into telomeric repeat-containing RNA (TERRA). Telomeric transcription has been documented in mammals, birds, zebra fish, plants and budding yeast. Here we show that the chromosome ends of Schizosaccharomyces pombe produce distinct RNA species. As with budding yeast and mammals, S. pombe contains G-rich TERRA molecules and subtelomeric RNA species transcribed in the opposite direction of TERRA (ARRET). Moreover, fission yeast chromosome ends produce two novel RNA species: C-rich telomeric repeat-containing transcripts (ARIA) and subtelomeric transcripts complementary to ARRET (αARRET). RNA polymerase II (RNAPII) associates with pombe chromosome ends in vivo and the telomeric factor Rap1 negatively regulates this association, as well as the cellular accumulation of RNA emanating from chromosome ends. We also show that the RNAPII subunit Rpb7 and the non-canonical poly(A) polymerases Cid12 and Cid14 are involved in the regulation of TERRA, ARIA, ARRET and αARRET transcripts. We confirm the evolutionary conservation of telomere transcription, and reveal intriguing similarities and differences in the composition and regulation of telomeric transcripts among model organisms.