Stimulation of uterine cell cytokine production by ovarian hormones.
Stimulation of uterine cell cytokine production by ovarian hormones.
复制标题
卵巢激素刺激子宫细胞细胞因子的产生。
DOI：
10.1111/j.8755-8920.2000.440103.x
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Kubik,C
中科院分区：
文献类型：
--
作者：
DeLoia,JA;Stewart-Akers,AM;Brekosky,J;Kubik,C
问题：虽然白细胞没有大量的卵巢类固醇激素受体，但它们在子宫内膜的数量随着月经周期的变化而变化。子宫内膜内的细胞类型表达白细胞吸引基因的可能性，以响应卵巢hormones.Method的研究黄体生成素激增后10天收集子宫内膜活检组织;细胞类型被分离，并单独培养5天的存在下，增加量的雌激素或孕激素。培养后，从细胞中收集RNA，并使用逆转录聚合酶链反应来确定单核细胞趋化蛋白（MCP）-1，2和3以及白细胞介素（IL）-12 p35和p40的相对基因表达水平。尽管子宫内膜基质和腺体都能够产生MCP mRNA，但相对于激素治疗，基因表达的稳态水平没有显著变化。IL-12基因的p35分子也发现了同样的结果;然而，p40亚基观察到了差异。结论在人类子宫内膜中，MCP和IL-12以外的趋化因子最有可能负责周期相关的白细胞募集。
PROBLEMAlthough leukocytes do not possess significant numbers of ovarian steroid hormone receptors, their numbers in the endometrium vary consistently, relative to the menstrual cycle. The possibility that cell types within the endometrium express leukocyte‐attracting genes in response to ovarian hormones was investigated.METHOD OF STUDYEndometrial biopsies were collected 10 days post‐leutinizing hormone surge; the cell types were separated and cultured individually for 5 days in the presence of increasing amounts of estrogen or progesterone. Following culture, RNA was collected from cells and reverse‐transcription‐polymerase chain reaction was used to determine relative levels of gene expression of monocyte chemotactic proteins (MCP)‐1, ‐2, and ‐3, and interleukin (IL)‐12p35 and p40.RESULTSAlthough both endometrial stroma and glands were able to make MCP mRNA, steady‐state levels of gene expression did not vary significantly relative to hormone treatment. The same was found for the p35 molecule of theIL‐12gene; however, differences were observed for the p40 subunit.CONCLUSIONSWithin the human endometrium, chemokines other than MCP and IL‐12 are most likely responsible for cycle‐related leukocyte recruitment.