Nodal coordinates the anterior-posterior patterning of germ layers and induces head formation in zebrafish explants
Nodal coordinates the anterior-posterior patterning of germ layers and induces head formation in zebrafish explants
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作者：
Tao Cheng;Yan-Yi Xing;Cong Liu;Yun-Fei Li;Ying Huang;Xiang Liu;Ying-Jie Zhang;Guo-Qin Zhao;Yang Dong;Xin-Xin Fu;Yi-Meng Tian;Li-Ping Shu;Sean Megason;Peng-Fei Xu
Much progress has been made toward generating analogs of early embryos, such as gastruloids and embryoids,.in vitro. However, methods for how to fully mimic the cell movements of gastrulation and coordinate.germ-layer patterning to induce head formation are still lacking. Here, we show that a regional Nodal gradient.applied to zebrafish animal pole explant can generate a structure that recapitulates the key cell movements of.gastrulation. Using single-cell transcriptome and in situ hybridization analysis, we assess the dynamics of the.cell fates and patterning of this structure. The mesendoderm differentiates into the anterior endoderm, prechordal.plate, notochord, and tailbud-like cells along an anterior-posterior axis, and an anterior-posteriorpatterned.head-like structure (HLS) progressively forms during late gastrulation. Among 105 immediate.Nodal targets, 14 genes contain axis-induction ability, and 5 of them induce a complete or partial head structure.when overexpressed in the ventral side of zebrafish embryos.
