Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora
Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora
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DOI：
10.1186/1471-2334-9-167
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发表时间：
2009-10-14
影响因子：
3.7
通讯作者：
Vaneechoutte, Mario
中科院分区：
文献类型：
--
作者：
El Aila, Nabil Abdullah;Tency, Inge;Vaneechoutte, Mario
背景：阴道微生物区系对于维持阴道健康和预防生殖道感染非常重要。直肠被认为是阴道econiche定植的主要来源。方法：为了确定直肠是否可以作为阴道econiche定殖的可能的细菌储存库，我们培养了妊娠35-37周的孕妇的阴道和直肠标本，用tRNA基因间长度多态性分析对分离株进行种间鉴定（tDNA-PCR），并对那些同时从阴道和直肠分离出相同菌种的受试者的分离株进行基因分型，在妊娠3537周时，从总共132名孕妇中的每一个收集一个阴道拭子和一个直肠拭子。拭子在哥伦比亚CNA琼脂和MRS琼脂上培养。对于每个受试者4个菌落被选择为每个两个网站，即8个菌落total.Results：在844株，可以通过tDNA-PCR鉴定，共有63种细菌，9（14%）只阴道，26（41%）只直肠，28（44%）在阴道和直肠。132份阴道样本中有121份（91.6%）和132份直肠样本中有51份（38.6%）乳酸杆菌阳性。L.卷曲乳杆菌是最常从阴道中分离出的乳杆菌（40%的受试者为阳性），其次是乳杆菌。jensenii（32%），L. gasseri（30%）和L.反对者（11%）。L.加氏乳杆菌是最常见的直肠分离乳杆菌（15%），其次是L. jensenii（12%），L. crispatus（11%）和L.共47例孕妇经阴道和直肠携带同种病毒。这导致了50对相同物种的阴道/直肠对，总共有8个不同的物种。对于50个物种对中的34个物种对（68%），具有相同基因型的分离株存在于阴道和直肠中，并且还建立了每个受试者物种内的高水平基因型多样性。可以得出结论，阴道和直肠微生物区系之间存在一定程度的对应关系，这些结果支持了直肠微生物区系作为细菌定殖库的假设，阴道木麻黄
Background: The vaginal microflora is important for maintaining vaginal health and preventing infections of the reproductive tract. The rectum has been suggested as the major source for the colonisation of the vaginal econiche.Methods: To establish whether the rectum can serve as a possible bacterial reservoir for colonisation of the vaginal econiche, we cultured vaginal and rectal specimens from pregnant women at 35-37 weeks of gestation, identified the isolates to the species level with tRNA intergenic length polymorphism analysis (tDNA-PCR) and genotyped the isolates for those subjects from which the same species was isolated simultaneously vaginally and rectally, by RAPD-analysis.One vaginal and one rectal swab were collected from a total of each of 132 pregnant women at 3537 weeks of gestation. Swabs were cultured on Columbia CNA agar and MRS agar. For each subject 4 colonies were selected for each of both sites, i.e. 8 colonies in total.Results: Among the 844 isolates that could be identified by tDNA-PCR, a total of 63 bacterial species were present, 9 (14%) only vaginally, 26 (41%) only rectally, and 28 (44%) in both vagina and rectum. A total of 121 (91.6%) of 132 vaginal samples and 51 (38.6%) of 132 rectal samples were positive for lactobacilli. L. crispatus was the most frequently isolated Lactobacillus species from the vagina (40% of the subjects were positive), followed by L. jensenii (32%), L. gasseri (30%) and L. iners (11%). L. gasseri was the most frequently isolated Lactobacillus species from the rectum (15%), followed by L. jensenii (12%), L. crispatus (11%) and L. iners (2%).A total of 47 pregnant women carried the same species vaginally and rectally. This resulted in 50 vaginal/rectal pairs of the same species, for a total of eight different species. For 34 of the 50 species pairs (68%), isolates with the same genotype were present vaginally and rectally and a high level of genotypic diversity within species per subject was also established.Conclusion: It can be concluded that there is a certain degree of correspondence between the vaginal and rectal microflora, not only with regard to species composition but also with regard to strain identity between vaginal and rectal isolates.These results support the hypothesis that the rectal microflora serves as a reservoir for colonisation of the vaginal econiche.