An improved porcine model of stable methacholine-induced bronchospasm
An improved porcine model of stable methacholine-induced bronchospasm
复制标题
稳定乙酰甲胆碱诱导的支气管痉挛的改进猪模型
DOI：
10.1007/s00134-002-1562-5
复制
发表时间：
2002
影响因子：
38.9
通讯作者：
G. Liistro
中科院分区：
文献类型：
--
作者：
C. Watremez;J. Roeseler;M. De Kock;T. Clerbaux;B. Detry;C. Veriter;M. Reynaert;P. Gianello;P. Jolliet;G. Liistro
摘要
目标。为了验证复制在人类中观察到的严重诱发支气管痉挛的病理生理学特征的动物模型，具有高水平的稳定性，允许使用多重惰性气体消除技术评估通风-灌流关系。
设计和布景。在动物研究实验室进行的实验研究。
受试者。研究了13头仔猪(3-4个月龄)，其中7头接受了完整的方案
干预措施。这些动物被麻醉并麻痹。机械通风以容量控制模式启动。调整呼吸参数以获得正常的碳酸血症，并在支气管痉挛期间保持恒定。乙酰甲胆碱通过同步雾化器给药，并逐步调整，以获得稳定的两倍峰值吸气压力。
测量和结果。在支气管痉挛之前和期间，获得心肺生理数据，包括肺力学评估和呼吸-灌流关系的测量。吸气峰压由19.7±2.9升至44.4±7.1cmH2O。后者在2小时内保持稳定。在所有动物中都观察到了呼吸力学、气体交换和呼吸-灌流分布的典型变化，这些变化在人类严重的支气管痉挛中观察到。
结论。目前的实验模型复制了严重人类支气管痉挛的一些生理病理特征，其稳定性应该有助于研究不同呼吸模式在急性重症哮喘中的作用。
Abstract
Objective. To validate an animal model replicating the pathophysiological characteristics of severe induced bronchospasm observed in humans, with a high level of stability permitting measurements such as the assessment of ventilation-perfusion relationships with the multiple inert gas elimination technique.
Design and setting. Experimental study in an animal research laboratory.
Subjects. 13 piglets (age 3–4 months) were studied and 7 underwent the complete protocol
Interventions. The animals were anesthetized and paralyzed. Mechanical ventilation was initiated in a volume-controlled mode. Ventilatory parameters were adjusted to obtain normocapnia and were maintained constant during the bronchospasm. Methacholine was administered via a synchronized nebulizer and progressively adjusted to obtain a stable twofold increase in peak inspiratory pressure.
Measurements and results. Cardiopulmonary physiological data including assessment of lung mechanics and measurement of ventilation-perfusion relationships were obtained before and during the bronchospasm. Peak inspiratory pressure increased from 19.7±2.9 to 44.4±7.1 cmH2O during the bronchospasm. The latter remained stable over 2 h. Respiratory mechanics, gas exchange, and ventilation-perfusion distribution changes typical of those observed in severe bronchospasm in humans were observed in all animals.
Conclusions. The present experimental model replicates some of the physiopathological characteristics of severe human bronchospasm, and its stability should facilitate studies of the effects of different ventilatory modes in the setting of acute severe asthma.
乙酰甲胆碱可导致犬支气管收缩、气体滞留和缺氧。
DOI：
10.1152/jappl.1987.63.1.262
发表时间：
1987
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Breen,PH;Becker,LJ;Ruygrok,P;Mayers,I;Long,GR;Leff,A;Wood,LD
通讯作者：
Wood,LD