The effect of 2,4-D and kinetin on dedifferentiation of petiole cells in Arabidopsis thaliana
The effect of 2,4-D and kinetin on dedifferentiation of petiole cells in Arabidopsis thaliana
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2,4-D和激动素对拟南芥叶柄细胞去分化的影响
DOI：
10.1007/s10535-012-0026-1
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发表时间：
2012-03-01
影响因子：
1.5
通讯作者：
Zhu, J.
中科院分区：
文献类型：
--
作者：
Li, F.;Cui, X.;Zhu, J.
植物激素是调节植物细胞脱分化不可缺少的因素。本文研究了不同浓度的2，4-二氯苯氧乙酸(2，4-D)和激动素(KIN)对拟南芥叶柄外植体愈伤组织形成前去分化细胞的解剖结构。结果表明，只有在含有2，4-D的条件下，维管束中薄壁细胞的细胞质才逐渐变得致密。在2，4-D存在下培养24 h后，WUSCHEL(WUS)基因在维管束的衍生细胞中表达，而单独用KIN培养的叶柄这些细胞中没有明显的信号。这些结果表明，2，4-D在拟南芥叶柄维管束细胞脱分化过程中起重要作用，而KIN对其无明显影响。
Phytohormones are indispensable factors regulating plant cell dedifferentiation. In this paper, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) were incorporated in the culture medium and the anatomy of dedifferentiated cells prior to callus formation from Arabidopsis thaliana petiole explants was examined. The results indicated that the cytoplasm of parenchyma cells in the vascular bundle gradually became denser with time of culture only if 2,4-D was included in the medium. The WUSCHEL (WUS) gene was expressed in derivative cells of the vascular bundle after culture for 24 h in the presence of 2,4-D and there was no obvious signal in these cells of cultured petioles with KIN alone. These results suggest that 2,4-D plays an important role in the process of dedifferentiation of vascular bundle cells in Arabidopsis petioles and KIN has no obvious effect on it.