Identification of quantitative trait loci associated with resistance to cucumber mosaic virus in Capsicum annuum
Identification of quantitative trait loci associated with resistance to cucumber mosaic virus in Capsicum annuum
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DOI：
10.1007/s001220100581
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发表时间：
2001-06-01
影响因子：
5.4
通讯作者：
Paran, I
中科院分区：
文献类型：
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作者：
Ben Chaim, A;Grube, RC;Paran, I
在种内辣椒群体中进行了黄瓜花叶病毒 (CMV) 抗性 QTL 分析。总共 180 个 F3 家族源自易感铃型品种 Maor 和抗性小果印度品系 Perennial 的杂交，并在美国和以色列使用两种病毒分离株进行的三项实验中接种了 CMV。主要采用RFLP和AFLP标记构建遗传图谱，并采用区间分析进行QTL检测。四个QTL与CMV抗性显着相关。还检测到两种双基因相互作用，涉及对 CMV 抗性有或没有个体影响的标记。在所有三个实验中均检测到控制观察到的表型变异 (cmv11.1) 的最大百分比 (16-33%) 的 QTL，并且还涉及双基因相互作用之一。该 QTL 与赋予对烟草花叶病毒 (TMV) 抗性的 L 基因座相关，证实了多年生植物对 CMV 抗性和对 TMV 易感性之间存在关联的早期轶事观察。对来自不相关种群 3990 的先进回交育种系进行了分析，以获取 CMV 抗性，以获取覆盖基因组的标记，从而能够识别从多年生植物渗入的基因组区域。其中四个基因渗入包括与 CMV 抗性 QTL 相关的区域。两个基因组区域中被鉴定为与 CMV 抗性 QTL 相关的标记也与果实重量 QTL 相关，证实了来自多年生植物和小果实重量的 CMV 抗性之间关联的额外育种观察结果。
QTL analysis for resistance to cucumber mosaic virus (CMV) was performed in an intraspecific Capsicum annuum population. A total of 180 F3 families were derived from a cross between the susceptible bell-type cultivar Maor and the resistant small-fruited Indian line Perennial and inoculated with CMV in three experiments carried out in the USA and Israel using two virus isolates. Mostly RFLP and AFLP markers were used to construct the genetic map, and interval analysis was used for QTL detection. Four QTL were significantly associated with resistance to CMV. Two digenic interactions involving markers with and without an individual effect on CMV resistance were also detected. The QTL controlling the largest percentage (16-33%) of the observed phenotypic variation (cmv11.1) was detected in all three experiments and was also involved in one of the digenic interactions. This QTL is linked to the L locus that confers resistance to tobacco mosaic virus (TMV), confirming earlier anecdotal observations of an association between resistance to CMV and susceptibility to TMV in Perennial. An advanced backcross breeding line from an unrelated population, 3990, selected for resistance to CMV was analyzed for markers covering the genome, allowing the identification of genomic regions introgressed from Perennial. Four of these introgressions included regions associated with QTL for CMV resistance. Markers in two genomic regions that were identified as linked to QTL for CMV resistance were also linked to QTL for fruit weight, confirming additional breeding observations of an association between resistance to CMV originating from Perennial and small fruit weight.