Monodispersed, molecularly imprinted polymers for cinchonidine by precipitation polymerization.
Monodispersed, molecularly imprinted polymers for cinchonidine by precipitation polymerization.
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DOI：
10.1016/j.talanta.2009.10.011
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发表时间：
2010-03
期刊：
影响因子：
6.1
通讯作者：
Yueqi Liu;Kaori Hoshina;J. Haginaka
中科院分区：
文献类型：
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作者：
Yueqi Liu;Kaori Hoshina;J. Haginaka
采用沉淀聚合法合成了三种单分散的辛可尼定分子印迹聚合物。以甲基丙烯酸（MAA）为功能单体，二乙烯基苯（DVB）为交联剂制备了MIP 1，并进一步加入甲基丙烯酸2-羟乙酯（HEMA）作为共聚单体制备了MIP 2。为了制备MIP 3，核-壳型MIP，单分散DVB均聚物，其通过沉淀聚合制备，用作核，并且将CD印迹MAA-DVB共聚物相涂覆到核上。合成的三种分子印迹聚合物均为单分散的微米级球形微珠。通过Scatchard分析和色谱研究考察了MIP 1 -3的结合特性和分子识别特性。CD与MIP 1的结合常数最高，与MIP 3的结合常数最低。模板分子，CD，是更多地保留比其立体异构体，辛可宁，在三个MIP，和立体分离因子38得到与MIP 3。
Three monodispersed, molecularly imprinted polymers (MIPs) for cinchonidine (CD) have been synthesized by precipitation polymerization. MIP1 was prepared using methacrylic acid (MAA) as a functional monomer and divinylbenzene (DVB) as a cross-linker and MIP2 was prepared with further addition of 2-hydroxyethyl methacrylate (HEMA) as a co-monomer. For the preparation of MIP3, core–shell type MIP, monodispersed DVB homopolymers, which are prepared by precipitation polymerization, were used as a core and CD-imprinted MAA–DVB copolymer phases were coated onto the core. Three MIPs synthesized gave monodispersed, spherical beads in micrometer sizes. The binding characteristics and molecular recognition properties of MIP1–3 were examined by Scatchard analysis and chromatographic studies. The association constant of CD with MIP1 was the highest among MIPs prepared, while that with MIP3 was the lowest. The template molecule, CD, was more retained than its stereoisomer, cinchonine, on the three MIPs, and the stereoseparation factor of 38 was obtained with MIP3.