Neuron-related blood inflammatory markers as an objective evaluation tool for major depressive disorder: An exploratory pilot case-control study
Neuron-related blood inflammatory markers as an objective evaluation tool for major depressive disorder: An exploratory pilot case-control study
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DOI：
10.1016/j.jad.2018.07.040
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发表时间：
2018-11-01
影响因子：
6.6
通讯作者：
Kanba, Shigenobu
中科院分区：
文献类型：
--
作者：
Kuwano, Nobuki;Kato, Takahiro A.;Kanba, Shigenobu
背景：神经炎症被认为是重性抑郁症（MDD）病理生理学的一个关键因素。外周血中神经源性外泌体（NDE）的分析最近被强调可以在不使用脑活检的情况下揭示脑疾病的病理生理学。目前，人类NDE研究需要大量的外周血来测量外泌体内的多种物质。方法：在健康对照组（HC）和未服药的MDD患者之间进行探索性的病例对照研究（每一个; N = 34），我们使用抗神经元抗体和抗CD 81抗体之间的新型夹心免疫测定法，用少量外周血寻找NDE相关的血液生物标志物结果：大多数神经元相关的血液生物标志物与CD 81（NDE）呈中强正相关，因此我们通过CD 81（每种生物标志物的数量/CD 81）标准化上述生物标志物来预测NDE相关的血液物质。MDD组IL 34/CD 81水平显著高于HC组。突触素（SYP），SYP/CD 81，肿瘤坏死因子受体1（TNFR 1）/CD 81与抑郁症和/或各种子symptoms.Limitations的严重程度呈正相关：我们实际上并没有提取NDE从外周blood.Conclusions：使用少量的外周血，我们已经成功地检测到可能的NDE相关的血液生物标志物。这是第一项研究表明，不仅SYP和TNFR 1，而且IL 34也是MDD患者的重要血液生物标志物。需要进一步的研究来评估本研究。
Background: Neuroinflammation is suggested to be a crucial factor in the pathophysiology of major depressive disorder (MDD). Analysis of neuron-derived exosomes (NDE) in peripheral blood has recently been highlighted to reveal the pathophysiology of brain diseases without using brain biopsy. Currently, human NDE studies require a considerable amount of peripheral blood to measure multiple substances inside exosomes. Previously, NDE-based clinical studies focusing on MDD have not been reported.Methods: As an exploratory pilot case-control study between healthy controls (HC) and drug-free MDD patients (each; N = 34), we searched for NDE-related blood biomarkers with a small amount of peripheral blood using a novel sandwich immunoassay between anti-neuron antibody and antibodies against CD81 (an exosome marker) and against other proteins related to neuroinflammation and synaptic functions.Results: Most neuron-related blood biomarkers had moderately to strongly positive correlation with CD81 (NDE), thus we normalized the above biomarkers by CD81 (quantity of each biomarker/CD81) to predict NDE-related blood substances. Interleukin 34 (IL34)/CD81 levels were significantly higher in MDD group compared to HC group. Synaptophysin (SYP), SYP/CD81, and tumor necrosis factor receptor 1 (TNFR1)/CD81 were positively correlated with severities of depression and/or various sub-symptoms.Limitations: We did not actually extract NDE from peripheral blood.Conclusions: Using a small amount of peripheral blood, we have successfully detected possible NDE-related blood biomarkers. This is the first study to suggest that not only SYP and TNFR1 but also IL34 are important blood biomarkers for patients with MDD. Further studies are warranted to evaluate the present study.