Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila
Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila
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DOI：
10.1016/j.aquaculture.2007.12.022
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发表时间：
2008-03-31
期刊：
影响因子：
4.5
通讯作者：
Jeney, Galina
中科院分区：
文献类型：
--
作者：
Ardo, Laszlo;Yin, Guojun;Jeney, Galina
研究了两种中草药黄芪和金银花与硼对尼罗罗非鱼非特异性免疫反应的影响。除了对照饮食（不含草药或硼）外，还使用了五种不同的饮食。其中含有0.1%的黄芪（含和不含0.05%的硼），0.1%的忍冬花（含或不含0.05%的硼）和两种草药的混合物（含0.05%的硼）。饲粮饲喂4周，测定呼吸爆发和血液白细胞吞噬活性；每周检测血浆溶菌酶、总蛋白、总免疫球蛋白水平。饲养四周后，鱼感染了嗜水气单胞菌，并记录了死亡率。结果表明，两种草药单独或联合饲喂罗非鱼可显著增强罗非鱼血液吞噬细胞的吞噬和呼吸爆发活性。它们对血浆溶菌酶水平有中等影响，对血浆总蛋白和总免疫球蛋白水平无影响。两种草药都降低了嗜水单胞菌感染后的死亡率。用草药和硼混合喂养的组死亡率最低。这些草药和硼的结合可以进一步提高病鱼的存活率。由此可见，饲料中添加黄芪、金银花提取物和补硼具有免疫刺激剂的作用，可增强养殖鱼类的免疫应答和抗病能力。(c) 2007 Elsevier B.V.版权所有
The effect of two Chinese medicinal herbs (Astragalus membranaceus and Lonicera japonica) and boron on non-specific immune response of Nile tilapia (Oreochromis niloticus) was investigated. Five diet variations in addition to a control diet (without herbs or boron) were used. These contained 0, 1 % Astragalus (with and without 0,05% boron), 0, 1 % Lonicera (with or without 0,05% boron) and a mixture of the two herbs with 0,05% boron. The diets were fed for four weeks, and respiratory burst and phagocytic activities of blood leukocytes; plasma lysozyme, total protein and total immunoglobulin level were measured weekly. After four weeks of feeding, fish were infected with Aeromonas hydrophila and mortalities were recorded. Results of this study showed that feeding tilapia with two herbs alone or in combination significantly enhanced phagocytic and respiratory burst activity of blood phagocytic cells. They had a moderate effect on the plasma lysozyme level and no effect on plasma total protein and total immunoglobulin level. Both herbs reduced the mortality following A. hydrophila infection. The lowest mortality was observed in the group fed with the combination of both herbs and boron. Combination of the herbs and boron could even more increase the survival rate of infected fish. It can be concluded that Astragalus and Lonicera extracts and boron supplementation added to fish feed can act as immunostimulants and enhance the immune response and disease resistance of cultured fish. (c) 2007 Elsevier B.V. All rights reserved.