Metabolomics reveals differences between three daidzein metabolizing phenotypes in adults with cardiometabolic risk factors.
Metabolomics reveals differences between three daidzein metabolizing phenotypes in adults with cardiometabolic risk factors.
复制标题
代谢组学揭示了具有心脏代谢危险因素的成年人中三种大豆苷元代谢表型之间的差异。
DOI：
10.1002/mnfr.201600132
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发表时间：
2017
影响因子：
5.2
通讯作者：
Steinberg,FranceneM
中科院分区：
文献类型：
--
作者：
Reverri,ElizabethJ;Slupsky,CarolynM;Mishchuk,DaryaO;Steinberg,FranceneM
范围大豆黄酮，大豆苷元，被肠道微生物群代谢为O-去甲基安哥拉辛（ODMA）和/或雌马酚。生产雌马酚被假定为一个促成因素的有益影响soy.Methods和resultsThis随机，对照，交叉设计使用了一个非靶向的代谢组学方法来评估不同的大豆苷元代谢的代谢概况。有心脏代谢风险因素的成人（n= 17）接受大豆坚果或对照食物4周，间隔2周洗脱期。干预前后以及干预之间未检测到显著差异。对大豆苷元代谢能力的检查显示了三组：仅ODMA生产者（n= 4），雌马酚+ ODMA生产者（n= 8）和非生产者（n= 5）。血清代谢组分析显示，非生产者可与仅ODMA生产者和雌马酚+ ODMA生产者区分开来。这些表型之间的差异与肥胖和代谢风险（甲硫氨酸、天冬酰胺和三甲胺）相关，其中雌马酚+ ODMA生产者的浓度较低，但矛盾的是促炎细胞因子较高。在尿液中，非生产者与ODMA生产者聚集在一起，与雌马酚+ ODMA生产者不同。尿代谢产物的配置文件显示显着较高的排泄富马酸和2-酮戊二酸，以及焦谷氨酸，丙氨酸，和肠道微生物代谢产物二甲胺在雌马酚+ ODMA producers.ConclusionThese结果强调，血清和尿液的代谢组是不同的基础上代谢的能力，马尿烷酮。
ScopeThe soy isoflavone, daidzein, is metabolized by gut microbiota toO‐desmethylangolensin (ODMA) and/or equol. Producing equol is postulated as a contributing factor for the beneficial effects of soy.Methods and resultsThis randomized, controlled, cross‐over design used an untargeted metabolomic approach to assess the metabolic profile of different daidzein metabolizers. Adults (n= 17) with cardiometabolic risk factors received soy nuts or control food for 4 weeks, separated by a 2‐week washout. No significant differences were detected pre‐ and postintervention and between interventions. Examination of the ability to metabolize daidzein revealed three groups: ODMA only producers (n= 4), equol + ODMA producers (n= 8), and nonproducers (n= 5). Analysis of the serum metabolome revealed nonproducers could be distinguished from ODMA‐only and equol + ODMA producers. Differences between these phenotypes were related to obesity and metabolic risk (methionine, asparagine, and trimethylamine) with equol + ODMA producers having lower concentrations, yet paradoxically higher pro‐inflammatory cytokines. In urine, nonproducers clustered with ODMA producers and were distinct from equol + ODMA producers. Urinary metabolite profiles revealed significantly higher excretion of fumarate and 2‐oxoglutarate, as well as pyroglutamate, alanine, and the gut microbial metabolite dimethylamine in equol + ODMA producers.ConclusionThese results emphasize that the serum and urine metabolomes are distinct based on the ability to metabolize isoflavones.