Regulation of mouse cytochrome P3-450 by the Ah receptor. Studies with a P3-450 cDNA clone.
Regulation of mouse cytochrome P3-450 by the Ah receptor. Studies with a P3-450 cDNA clone.
复制标题
Ah 受体对小鼠细胞色素 P3-450 的调节。
DOI：
10.1021/bi00320a016
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
D. Nebert
中科院分区：
文献类型：
--
作者：
R. Tukey;D. Nebert
The Ah locus in the C57BL/6N mouse regulates at least two cytochrome P-450 gene products, termed in the mouse P1-450 and P3-450; these two enzymes are so named because each is responsible for the highest turnover number for the substrates benzo[a]pyrene and acetanilide, respectively. A cDNA library was prepared in pBR322 from sucrose gradient fractionated total liver poly(A+)-enriched RNA (approximately 20 S) from 2,3,7,8-tetrachlorodibenzo-p-dioxin- (TCDD) treated C57BL/6N (Ahb/Ahb) mice. Differential colony hybridization screening, with [32P]cDNA probes derived from total liver mRNA of both TCDD-treated and control C57BL/6N mice, yielded pP(3)450-21 (1710 base pair) and pP(1)450-57 (1770 base pair) cDNA clones. pP(1)450-57 was found to have 690 base pairs 5'-ward of the original P1-450 cDNA cloned in this laboratory. Restriction maps of pP(3)450-21 and pP(1)450-57 are markedly different and clearly are derived from separate genes. By means of hybridization-translation-arrest experiments, anti-(P3-450) precipitates the translation product (Mr approximately equal to 55000) of mRNA specifically hybridizing to pP(3)450-21. It is also shown that hybridization-translation-arrest experiments using polyclonal antibodies are not specific for proof of a P-450 cDNA clone. pP(3)450-21 was used to probe liver mRNA from Ahb/Ahb, Ahb/Ahd, and Ahd/Ahd mice treated with 3-methylcholanthrene, beta-naphthoflavone, aroclor 1254, isosafrole, low TCDD, or high TCDD. These genetic data rigorously demonstrate control of the P3-450 (20S) mRNA induction process by the Ah receptor. pP(3)450-21 fragments hybridized to TCDD-induced C57BL/6N mRNA and to a portion of the cloned 5' end of the P1-450 gene from a mouse MOPC 41 plasmacytoma library.(ABSTRACT TRUNCATED AT 250 WORDS)
钙调蛋白的结晶和初步 X 射线研究。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cook,WJ;Dedman,JR;Means,AR;Bugg,CE
通讯作者：
Bugg,CE
克隆的细胞色素 P-450 cDNA。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kumar,A;Raphael,C;Adesnik,M
通讯作者：
Adesnik,M
包含卵清蛋白多基因家族的染色质结构域的 5 和 3 结构边界的定义。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lawson,GM;Knoll,BJ;March,CJ;Woo,SL;Tsai,MJ;O'Malley,BW
通讯作者：
O'Malley,BW