Discovery of osteoblast-associated genes using cDNA microarrays
Discovery of osteoblast-associated genes using cDNA microarrays
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DOI：
10.1016/s8756-3282(01)00699-8
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发表时间：
2002-03-01
期刊：
影响因子：
4.1
通讯作者：
Seth, A
中科院分区：
文献类型：
--
作者：
Raouf, A;Seth, A
成骨细胞成熟是一个复杂的过程，涉及不同的基因型变化，伴随着特定的表型改变。为了鉴定成骨细胞中新的骨相关基因，我们使用了IncyteGenomics， Inc. （St. Louis, Mo）的高密度小鼠GEM1微阵列基因芯片。我们检测了超过8700个基因在MC3T3-E1增殖（第3天）和矿化（第34天）阶段的表达谱。超过8600个基因提供了可测量的信号。在这些基因中，252个在第3天和第34天被发现有差异表达。大量这些基因以前从未在成骨细胞发育的背景下被识别。在增殖成骨细胞中，大约60%的显性表达基因是生长相关基因，如TACC3和Pr22。TIS21/BTG2和一个新基因EST350的表达在分化阶段（第12天）达到峰值，表明它们可能在成骨细胞分化中发挥重要作用。矿化期显性表达的基因主要是信号转导基因和细胞外基质（ECM）蛋白，如lumican和cystatin-C。值得注意的是，第3天没有检测到lumican的表达，这表明该基因可能是有丝分裂后成骨细胞的重要标志物。建立这些及其他MC3T3-E1成骨细胞发育不同阶段基因的表达谱，将使我们能够区分成骨细胞生物学不同阶段的分子事件。（Bone 30:463-471; 2002） (C) 2002年Elsevier Science Inc.出版。版权所有。
Osteoblast maturation is a complex process and involves distinct genotypic changes that are accompanied by specific phenotypic alterations. To identify new bone-related genes in osteoblasts we utilized the high-density mouse GEM1 microarray gene chip from IncyteGenomics, Inc. (St. Louis, Mo). We examined the expression profiles of over 8700 genes during the proliferation (day 3) and the mineralization (day 34) phases of MC3T3-E1 development. More than 8600 genes provided measurable signals. Of these genes, 252 were found to be differentially expressed on days 3 and 34. A large number of these genes have never been previously recognized in the context of osteoblast development. Approximately, 60% of the genes with expressions that were dominant in proliferating osteoblasts consisted of growth-related genes such as TACC3 and Pr22. The expressions of TIS21/BTG2, and a novel gene EST350, were found to peak during the differentiation phase (day 12), suggesting that they may play important roles in osteoblast differentiation. The majority of the genes with expressions that were dominant during the mineralization phase consisted of signal transduction genes and extracellular matrix (ECM) proteins such as lumican and cystatin-C. It is significant that lumican expression could not be detected on day 3, which indicates that this gene may serve as an important marker of postmitotic osteoblasts. The establishment of the expression profiles of these and other genes with various phases of MC3T3-E1 osteoblast development will allow us to distinguish the molecular events at different phases of osteoblast biology. (Bone 30:463-471; 2002) (C) 2002 by Elsevier Science Inc. All rights reserved.