I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions
I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions
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DOI：
10.1021/pr050225e
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发表时间：
2005-09-01
影响因子：
4.4
通讯作者：
Chait, BT
中科院分区：
文献类型：
--
作者：
Tackett, AJ;DeGrasse, JA;Chait, BT
通过亲和标记的蛋白质分离蛋白质复合物提供了一个强大的工具，用于研究生物系统，但该技术往往是妥协的非特异性相互作用的蛋白质的共富集。我们描述了一种新的技术（I-DIRT），区分污染物从真正的相互作用在免疫纯化，克服这个最具挑战性的问题，在定义蛋白质复合物。I-DIRT技术在研究生物体系中可代谢标记的蛋白质复合物方面具有广泛的应用价值。
Isolation of protein complexes via affinity-tagged proteins provides a powerful tool for studying biological systems, but the technique is often compromised by co-enrichment of nonspecifically interacting proteins. We describe a new technique (I-DIRT) that distinguishes contaminants from bona fide interactors in immunopurifications, overcoming this most challenging problem in defining protein complexes. I-DIRT will be of broad value for studying protein complexes in biological systems that can be metabolically labeled.