Elevation of N-(carboxymethyl)valine residue in hemoglobin of diabetic patients. Its role in the development of diabetic nephropathy.
Elevation of N-(carboxymethyl)valine residue in hemoglobin of diabetic patients. Its role in the development of diabetic nephropathy.
复制标题
糖尿病患者血红蛋白中N-(羧甲基)缬氨酸残基升高。
DOI：
10.2337/diacare.24.5.891
复制
发表时间：
2001
期刊：
影响因子：
16.2
通讯作者：
I. Maruyama
中科院分区：
文献类型：
--
作者：
T. Uchimura;K. Nakano;T. Hashiguchi;H. Iwamoto;K. Miura;Y. Yoshimura;N. Hanyu;K. Hirata;M. Imakuma;Y. Motomiya;I. Maruyama
客观的
OBJECTIVE
Advanced glycation end products (AGEs) are a risk factor for diabetic complications. We have developed an assay method for N-(carboxymethyl)valine (CMV) of the hemoglobin (CMV-Hb), which is an AGE generated from HbA1c. Herein we describe the clinical utility of CMV-Hb measurement for the diagnosis of diabetic nephropathy
RESEARCH DESIGN AND METHODS
BALB/c mice were immunized with carboxy-methylated Hb and monoclonal antibody raised against CMV-Hb. This antibody was characterized by a surface plasmon resonance. We developed a latex immunoassay using the antibody and measured CMV-Hb from erythrocytes in type 2 diabetic patients and healthy control subjects (age 64.6 +/- 12.0 vs. 61.1 +/- 13.2 years, NS: HbA1c 69 +/- 1.5 vs. 5.2 +/- 0.4%, P < 0.0001).
RESULTS
A monoclonal antibody against CMV-Hb beta-chain NH2-terminal and an assay method for measurement for CNMV-Hb were both developed in our laboratory. CMV-Hb levels were significantly greater in the diabetic patients than in the control subjects (18.2 +/- 6.9 vs. 12.7 +/- 0.9 pmol CMV/mg Hb, P < 0.0001). No correlation was found between CMV-Hb and HbA1c or CMV-Hb and glycated albumin. Levels of CMV-Hb increased as the diabetic nephropathy progressed.
CONCLUSIONS
We established an assay method for CMV-Hb and confirmed the presence of CMV-Hb in circulating erythrocytes. CMV-Hb was more prevalent in diabetic patients than in healthy subjects. Furthermore, it was significantly higher in patients with diabetic nephropathy, suggesting that the presence of CMV-Hb may be a valuable marker for the progression of diabetic nephropathy.
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DOI：
10.1056/nejm198805193182007
发表时间：
1988-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
M. Brownlee;A. Cerami;H. Vlassara
通讯作者：
M. Brownlee;A. Cerami;H. Vlassara
影响因子：
15.9
作者：
SCHMIDT, AM;HORI, O;STERN, D
通讯作者：
STERN, D
DOI：
10.1016/0167-4838(94)00209-y
发表时间：
1995-02-22
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
影响因子：
--
作者：
PORTEROOTIN, M;NAGARAJ, RH;MONNIER, VM
通讯作者：
MONNIER, VM
影响因子：
15.9
作者：
MCCANCE, DR;DYER, DG;LYONS, TJ
通讯作者：
LYONS, TJ
影响因子：
2.9
作者：
DUNN, JA;PATRICK, JS;BAYNES, JW
通讯作者：
BAYNES, JW