Transcriptomic analysis reveals key genes associated with the biosynthesis regulation of phenolics in fresh-cut pitaya fruit (Hylocereus undatus)
Transcriptomic analysis reveals key genes associated with the biosynthesis regulation of phenolics in fresh-cut pitaya fruit (Hylocereus undatus)
复制标题
转录组分析揭示了与鲜切火龙果（Hylocereus undatus）酚类生物合成调控相关的关键基因
DOI：
10.1016/j.postharvbio.2021.111684
复制
发表时间：
2021-08-10
影响因子：
7
通讯作者：
Zheng, Yonghua
中科院分区：
文献类型：
--
作者：
Li, Xiaoan;Li, Bingru;Zheng, Yonghua
伤害胁迫诱导火龙果果实中酚类物质的积累。本研究旨在通过转录组学和生物信息学分析，阐明伤诱导鲜切火龙果酚类物质生物合成的可能分子机制。创伤应激激活了与酚类物质生物合成相关的代谢途径，包括苯丙素途径和类黄酮途径等次生代谢，乙烯、活性氧和茉莉酸等信号分子代谢，糖酵解、磷酸戊糖途径和莽草酸途径等初级代谢。加权基因共表达网络分析和进化关系分析表明，1个HuMYB、3个HubHLH、7个HuAP 2-EREBP可能是参与伤害诱导的火龙果酚类物质生物合成调控的转录因子。这一结果在转录水平上验证了损伤胁迫诱导鲜切火龙果果实己糖库转化为酚类物质积累提供必需碳骨架的研究，为进一步研究转录因子在火龙果果实损伤反应中的作用提供了重要的遗传信息。
Wounding stress induces phenolic accumulation in pitaya fruit (Hylocereus undatus). This study aimed to elucidate the possible molecular mechanism underlying the wound-induced phenolic biosynthesis in fresh-cut pitaya fruit based on transcriptomic and bioinformatic analysis. Wounding stress induced the activation of metabolic pathways associated with phenolic biosynthesis, including secondary metabolism such as phenylpropanoid pathway and flavonoid pathway, signaling molecules metabolism such as ethylene, reactive oxygen species and jasmonic acid, and primary metabolism such as glycolysis, pentose phosphate pathway and shikimate pathway. Moreover, weighted gene coexpression network analysis and evolutionary relationship analysis revealed that 1 HuMYB, 3 HubHLHs, 7 HuAP2-EREBPs could be identified as putative transcription factors participating in the regulation of wound-induced phenolic biosynthesis in pitaya fruit. These findings validated previous study that wounding stress induces the conversion of hexose pool to supply essential carbon skeletons for the phenolic accumulation in fresh-cut pitaya fruit in transcriptional level and provide important and useful genetic information for further studies on the functions of transcription factors in wounding response in pitaya fruit.