Synthesis of papain-polyacrylamide hydrogel microspheres and their catalytic application
Synthesis of papain-polyacrylamide hydrogel microspheres and their catalytic application
复制标题
木瓜蛋白酶-聚丙烯酰胺水凝胶微球的合成及其催化应用
DOI：
10.1039/d1nj02551a
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发表时间：
2021
影响因子：
3.3
通讯作者：
Wang Yi
中科院分区：
文献类型：
--
作者：
Ma Yuan;Li Yao;Fei Xu;Tian Jing;Xu Longquan;Wang Yi
固定化酶技术虽然有效地提高了酶的重复使用性和稳定性，但共价键或/和电荷相互作用的引入导致酶的二级结构发生变化，从而降低了催化活性。本文提出了一种温和有效的酶固定化方法。设计并合成了一种粒径可控的水凝胶微球作为固定化酶载体。木瓜蛋白酶通过氢键作用固定在微球的表面和孔内，对酶的二级结构无不良影响。木瓜蛋白酶-聚丙烯酰胺水凝胶微球的酶活为游离木瓜蛋白酶的106.41%。固定化木瓜蛋白酶循环10次后仍保持游离酶活力的59.60%。实际催化应用结果证明，所制备的木瓜蛋白酶-聚丙烯酰胺水凝胶微球非常适合于牛乳中过敏原蛋白的催化水解，且不引入任何其它杂蛋白。
Although immobilized enzyme technologies effectively improve the reusability and stability of an enzyme, the introduction of covalent bonds or/and charge interaction causes the change for the secondary structure of an enzyme, thereby reducing the catalytic activity. In this work, a mild and efficient strategy for enzyme immobilization is proposed. A size-controlled hydrogel microsphere was designed and synthesized as an immobilized enzyme carrier. Papain was immobilized on the surface and pores of hydrogel microspheres by hydrogen bonding interactions, which have no adverse effect on the secondary structure of an enzyme. The activity of the papain–polyacrylamide hydrogel microsphere was 106.41% of that of free papain. In addition, the immobilized papain maintained 59.60% of free papain activity after 10 cycles. The results of practical catalytic application proved that the obtained papain–polyacrylamide hydrogel microspheres were very suitable for catalytic hydrolysis of allergen protein in cow's milk without introducing any other hetero protein.