In Situ Antibody Detection and Charge Discrimination Using Aqueous Stable Pentacene Transistor Biosensors
In Situ Antibody Detection and Charge Discrimination Using Aqueous Stable Pentacene Transistor Biosensors
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DOI：
10.1021/ja107088m
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发表时间：
2011-02-23
影响因子：
15
通讯作者：
Knoll, Wolfgang
中科院分区：
文献类型：
--
作者：
Khan, Hadayat Ullah;Jang, Junhyuk;Knoll, Wolfgang
基于并五苯的有机薄膜晶体管被用于创建用于选择性抗体检测的高灵敏度实时电子传感器。将牛血清白蛋白共价连接到修饰的并五苯表面，以选择性地检测无标记的单克隆抗BSA。这些传感器在pH 7下显示出（1.1 +/-3）× 10（7）M-1的高亲和常数（K-A），这比用高灵敏度表面等离子体共振光谱检测系统获得的那些高1个数量级。此外，在不同的pH值下，实现了高度的歧视在混合抗BSA电荷。这种使用有机晶体管传感平台在水性缓冲溶液中快速、无标记、实时检测纳米级生物分子的示范，除了区分可电离基团的存在外，还将对高性能微阵列产生重大影响。
Pentacene-based organic thin-film transistors were used to create highly sensitive, real-time electronic sensors for selective antibody detection. Bovine serum albumin was covalently attached to a modified pentacene surface to selectively detect the label free monoclonal antiBSA. These sensors displayed a high affinity constant (K-A) of (1.1 +/- 3) x 10(7) M-1 at pH 7, which is 1 order of magnitude higher than those obtained with a highly sensitive surface plasmon resonance spectroscopy detection system. Furthermore, a high degree of discrimination in the hybrid antiBSA charges was achieved at different pH values. This demonstration of fast, label-free, real-time detection of nanoscale biomolecules in aqueous buffer solutions using the organic transistor sensing platform will have a significant impact on high-performance microarrays in addition to discriminating the presence of ionizable groups.