Two DNA nanomachines map pH changes along intersecting endocytic pathways inside the same cell
Two DNA nanomachines map pH changes along intersecting endocytic pathways inside the same cell
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DOI：
10.1038/nnano.2013.92
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发表时间：
2013-06-01
影响因子：
38.3
通讯作者：
Krishnan, Yamuna
中科院分区：
文献类型：
--
作者：
Modi, Souvik;Nizak, Clement;Krishnan, Yamuna
DNA是活细胞中分子传感的多功能支架，并且DNA纳米器件的各种细胞应用已经被证明。然而，在同一活细胞内同时使用不同的DNA纳米器件仍然是一个挑战。在这里，我们表明，两个不同的DNA纳米机器可以同时用于映射pH梯度沿着两个不同的，但相交的细胞进入途径。这两个纳米机器，这是分子编程通过不同的途径进入细胞，可以映射pH值的变化在明确界定的亚细胞环境沿着这两个途径在同一个细胞内。我们应用这些纳米机器来探测早期内涵体和trans-Golgi网络的pH值，在真实的时间。当依次或同时递送时，两种纳米机器都定位于并独立捕获它们所设计的细胞器的pH值。DNA纳米器件在生命系统中的成功运作对各种背景下的传感和治疗具有重要意义。
DNA is a versatile scaffold for molecular sensing in living cells, and various cellular applications of DNA nanodevices have been demonstrated. However, the simultaneous use of different DNA nanodevices within the same living cell remains a challenge. Here, we show that two distinct DNA nanomachines can be used simultaneously to map pH gradients along two different but intersecting cellular entry pathways. The two nanomachines, which are molecularly programmed to enter cells via different pathways, can map pH changes within well-defined subcellular environments along both pathways inside the same cell. We applied these nanomachines to probe the pH of early endosomes and the trans-Golgi network, in real time. When delivered either sequentially or simultaneously, both nanomachines localized into and independently captured the pH of the organelles for which they were designed. The successful functioning of DNA nanodevices within living systems has important implications for sensing and therapies in a diverse range of contexts.