Hyperplastic foci in precancerous rat liver: light microscopic and electron microscopic study.
Hyperplastic foci in precancerous rat liver: light microscopic and electron microscopic study.
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癌前大鼠肝脏增生灶：光学显微镜和电子显微镜研究。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
A. Timme
中科院分区：
文献类型：
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作者：
A. Timme
4-在通过饮食中加入柠檬酸铁诱导肝铁质沉着症后，向Wistar衍生的雄性白化病大鼠喂食二甲基氨基偶氮苯。肝细胞出现无铁病灶，这一特征使其能够在棕色实质中肉眼识别。五个这样的病变，每个直径为1毫米或更小，进行了研究，通过光学和电子显微镜。病灶中的细胞比病灶周围的细胞大，具有颗粒状和中度嗜碱性细胞质。在超微结构上，细胞与正常肝细胞非常相似。它们具有发达的粗面内质网，大量的游离核糖体，过氧化物酶体，胆小管，和细胞质连接复合物，但只有少量的糖原储存观察。偶尔铁蛋白负载溶酶体持续在一些细胞。这些病灶被认为是增生性的。它们可能演变成嗜碱性或空泡型增生结节。这些病灶应与由分化极差的细胞组成的嗜碱性病灶清楚区分。
4-Dimethylaminoazobenzene was fed to Wistar-derived, male, albino rats after hepatic siderosis had been induced by including ferric citrate in the diet. Iron-free foci of hepatocytes developed and this characteristic enabled them to be recognized macroscopically in the brown parenchyma. Five such lesions, each 1 mm or less in diameter, were studied by light and electron microscopy. The cells in the foci were larger than those surrounding the foci and had a granular and moderately basophilic cytoplasm. Ultrastructurally, the cells closely resembled normal hepatocytes. They possessed well-developed rough endoplasmic reticulum, numerous free ribosomes, peroxisomes, bile canaliculi, and cytoplasmic junctional complexes, but only small stores of glycogen were observed. Occasional ferritin-laden lysosomes persisted in some cells. These foci were regarded as hyperplastic. Possibly, they evolved into hyperplastic nodules either of the basophilic or vacuolated type. These foci should be clearly distinguished from hyperbasophilic foci that consisted of very poorly differentiated cells.