NKB1 - A NATURAL-KILLER-CELL RECEPTOR INVOLVED IN THE RECOGNITION OF POLYMORPHIC HLA-B MOLECULES
NKB1 - A NATURAL-KILLER-CELL RECEPTOR INVOLVED IN THE RECOGNITION OF POLYMORPHIC HLA-B MOLECULES
复制标题
DOI：
10.1084/jem.180.2.537
复制
发表时间：
1994-08-01
影响因子：
15.3
通讯作者：
LANIER, LL
中科院分区：
文献类型：
--
作者：
LITWIN, V;GUMPERZ, J;LANIER, LL
自然杀伤（NK）细胞可杀伤缺乏主要组织相容性复合体（MHC）I类抗原表达的正常及转化的造血细胞。人NK细胞克隆可裂解HLA阴性的爱泼斯坦 - 巴尔病毒（EBV）转化的B淋巴母细胞系（B - LCL），而通过用某些HLA - A、 - B或 - C等位基因转染靶细胞可抑制这种裂解作用。从个体建立的NK细胞克隆在HLA识别上表现出克隆异质性，且单个NK克隆可识别多个等位基因。我们描述了一种由单克隆抗体DX9鉴定的针对某些HLA - B等位基因（例如，HLA - B*5101和 - B*5801）的潜在人NK细胞受体（NKB1）。NKB1是一种70 - kD的糖蛋白，在一部分NK细胞和NK细胞克隆上表达。DX9单克隆抗体（mAb）特异性地抑制NK细胞克隆与转染了某些HLA - B等位基因的B - LCL靶细胞之间的相互作用，但不影响对HLA - A或HLA - C抗原的识别。单个NK细胞克隆可独立识别转染了HLA - B或HLA - C抗原的B - LCL靶细胞；然而，DX9 mAb仅影响与表达某些HLA - B等位基因的转染细胞的相互作用。这些发现证明了参与HLA - B识别的NK细胞受体的存在，并暗示单个NK克隆上存在多种MHC受体。
Natural killer (NK) cells kill normal and transformed hematopoietic cells that lack expression of major histocompatibility complex (MHC) class I antigens. Lysis of HLA-negative Epstein Barr virus-transformed B lymphoblastoid cell lines (B-LCL) by human NK cell. clones can be inhibited by transfection of the target cells with certain HLA-A, -B, or -C alleles. NK cell clones established from an individual demonstrate clonal heterogeneity in HLA recognition and a single NK clone can recognize multiple alleles. We describe a potential human NK cell receptor (NKB1) for certain HLA-B alleles (e.g., HLA-B*5101 and-B*5801)identified by the mAb DX9. NKB1 is a 70-kD glycoprotein that is expressed on a subset of NK cells and NK cell clones. DX9 monoclonal antibody (mAb) specifically inhibits the interaction between NK cell clones and B-LCL targets transfected with certain HLA-B alleles, but does not affect recognition of HLA-A or HLA-C antigens. An individual NK cell clone can independently recognize B-LCL targets transfected with HLA-B or HLA-C antigens; however, DX9 mAb only affects interaction with transfectants expressing certain HLA-B alleles. These findings demonstrate the existence of NK cell receptors involved in the recognition of HLA-B and imply the presence of multiple receptors for MHC on an individual NK clone.